We narrowed to 10,181 results for: Set

1. pSEED-Moontag:HA

   Plasmid

   #219648

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   MoonTag:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-EYFP:HA

   Plasmid

   #219656

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   EYFP:HA SEED Cassette
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDC.118

   Plasmid

   #227188

   Purpose

   T7 IPTG-driven Cas13a

   Depositor

   Seth Shipman

   Article

   Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

   Insert

   LbuCas13a
   

   Expression

   Bacterial

   Available Since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJBM2

   Plasmid

   #225289

   Purpose

   E. coli-Campylobacter shuttle vector; KanR

   Depositor

   Robert Moore

   Article

   McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

   Insert

   Replication elements from pCC311 for pJBM2
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLP5

   Plasmid

   #209960

   Purpose

   Entry vector to store Level 0* resistance cassette parts

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pLPC-C-SF-TAP-DEST-TLK1- M566T

   Plasmid

   #218831

   Purpose

   Expresses C-terminal Strep-FLAG-tagged M566T human TLK1 variant 5 in a retrovirus vector.

   Depositor

   Travis Stracker

   Insert

   TLK1 M566T (TLK1 Human)
   

   Tags

   FLAG-STREP

   Expression

   Mammalian

   Mutation

   M566T

   Promoter

   CMV

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCL194

   Plasmid

   #222225

   Purpose

   Expresses site 1/Cas9/Eco1 RT in HIS3 locus.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   site 1/Cas9/Eco1 RT
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSCL195

   Plasmid

   #222226

   Purpose

   Expresses site 2/Cas9/Eco1 RT in HIS3 locus.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   site 2/Cas9/Eco1 RT
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSCL368

   Plasmid

   #222227

   Purpose

   Expresses site 3/Cas9/Eco1 RT in HIS3 locus.

   Depositor

   Seth Shipman

   Article

   Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   site 3/Cas9/Eco1 RT
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0.425

    Plasmid

    #203944

    Purpose

    Up Flank. Up flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    Cas12a UP
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pC0.426

    Plasmid

    #203945

    Purpose

    Down Flank. Down flanking region of RSF1010-Cas12a vector for introducing gRNA expression cassette and swapping out the AbR.

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Insert

    Cas12a DOWN
    

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgRNA TR KO 2

    Plasmid

    #207610

    Purpose

    sgRNA 2 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    TCAGGCCGCAGGAAGAGGAA
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA TR KO 1

    Plasmid

    #207609

    Purpose

    sgRNA 1 to knockout TR by replacement with a PuroR cassette

    Depositor

    Jens Schmidt

    Article

    Klump et al Cell Rep. 2023 Jun 1;42(6):112577. doi:

    Insert

    ACCCTAACTGAGAAGGGCGT
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV and U6

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUPD2-HR1bR181A R185A-GFP-L (B3-B4)

    Plasmid

    #211857

    Purpose

    Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Tags

    GFP

    Mutation

    R181A R185A

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. SOX2-P2A-NLS-mClover3-IRES-Puro

    Plasmid

    #215544

    Purpose

    Donor plasmid for knock-in P2A-NLS-mClover3 into the human SOX2 locus

    Depositor

    Kai Wang

    Insert

    SOX2 homologous recombination arms with IRES-Puro selection cassette (SOX2 Human)
    

    Use

    CRISPR

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDT2

    Plasmid

    #199119

    Purpose

    circular transcription template

    Depositor

    Jeff Gelles

    Article

    Inlow et al Proc Natl Acad Sci U S A. 2023 Jul 11;12

    Insert

    lambda PR' - repeat cassette - E. coli rpoB - lambda TR'
    

    Expression

    Bacterial

    Mutation

    mutated 5′-CTGGAGTGCG-3′ to 5′-CTGGAGACCG-3′ to …

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. NK621

    Plasmid

    #196405

    Purpose

    Strong expression of firefly luciferase codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. BASIC_SEVA_69.11

    Plasmid

    #194214

    Purpose

    BASIC DNA assembly vector

    Depositor

    Paul Freemont

    Article

    Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

    Insert

    mScarlet counter-selection cassette
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 26, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. XLone-mARG2

    Plasmid

    #173792

    Purpose

    Mammalian acoustic reporter gene 1 cassette 2 on XLone plasmid

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpN, F G L S K J U
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    TRE3G promoter

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. IRE-miR21mutant-SunTag-TREAT

    Plasmid

    #196933

    Purpose

    SunTag cassette was added in the coding region in the miR21mutant -IRE-TREAT reporter.

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Expression

    Bacterial and Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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