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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 33,230 results for: his

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Showing: 7641 - 7660 of 33230 results

  1. pBad-mAmetrine

    Plasmid
    #18083
    Purpose
    Bacterial expression of mAmetrine fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Insert
    mAmetrine fluorescent proteins
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Available Since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  2. pCoofy44

    Plasmid
    #55185
    Purpose
    Insect expression vector for parallel SLIC cloning containing N-terminal 10x His and SumoStar tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    10x His, 10x His-Stop-OneStrep-Stop-S-tag-Stop-HP…
    Expression
    Bacterial
    Promoter
    pPH (Polyhedrin promoter)
    Available Since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    55185-plasmid-map-sequence-id-85559

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  3. pcDNA3-IGFBP5-V5

    Plasmid
    #11608
    Depositor
    Steven Johnson
    Insert
    Insulin Growth Factor Binding Protein 5 (IGFBP5 Human)
    Tags
    His and V5
    Expression
    Mammalian
    Mutation
    no mutations
    Available Since
    July 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  4. Sc hk/P-RFA3-pCDFDuet

    Plasmid
    #240743
    Purpose
    Over-express his(10)-PKA kinase motif-PreScission protease site-Sc RFA3 in E. coli
    Depositor
    Michael O'Donnell
    Insert
    hk/P-RFA3 (RFA3 Budding Yeast)
    Tags
    His(10) followed by PKA kinase motif follwed by P…
    Expression
    Bacterial
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240743-plasmid-map-sequence-id-482370

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  5. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Available Since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  6. ppie.001.301.W257A

    Plasmid
    #137652
    Purpose
    Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.
    Depositor
    Tara Davis, Melissa Jurica
    Insert
    ppie (PPIE Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    001-301-W257A
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137652-plasmid-map-sequence-id-381379

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  7. PRPF4.001.522.K45A.pc4

    Plasmid
    #137648
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-K45A-pc4
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137648-plasmid-map-sequence-id-381373

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  8. PRPF4.001.522.Y32A.pc4

    Plasmid
    #137646
    Purpose
    Expresses nuclear localization signal (NLS), C-terminal myc-(His)6 fusion of human gene or portion of gene in human cell culture lines. pcDNA4.1-based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    NLS and myc-(His)6
    Expression
    Mammalian
    Mutation
    001-522-Y32A-pc4
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137646-plasmid-map-sequence-id-381367

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  9. FtHAP-pNIC-Bsa4

    Plasmid
    #86778
    Purpose
    Expresses Francisella tularensis Histidine Acid Phosphatase under T7 with HIS tag
    Depositor
    Josh Beckham
    Insert
    Histidine Acid Phosphatase
    Tags
    6x His tag
    Expression
    Bacterial
    Available Since
    Feb. 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    86778-plasmid-map-sequence-id-462633

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  10. pEU-Gm23

    Plasmid
    #53738
    Purpose
    Used for in vitro transcription/translation of soybean His-tagged arginyl-tRNA synthetase in the Cell Free Sciences system
    Depositor
    Gabor Igloi
    Insert
    arginyl-tRNA synthetase
    Use
    Synthetic Biology
    Tags
    His tag
    Promoter
    SP6
    Available Since
    July 31, 2014
    Availability
    Academic Institutions and Nonprofits only
    53738-plasmid-map-sequence-id-83944

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  11. P48

    Plasmid
    #212155
    Purpose
    Plasmid expressing 6x His tagged secondary alcohol dehydrogenase fused with superfolder green fluorescent protein at N terminus under constitutive promoter PgyrA control.
    Depositor
    Kang Zhou
    Insert
    superfolder green fluorescent protein, secondary alcohol dehydrogenase
    Tags
    6x His
    Expression
    Bacterial
    Promoter
    PgyrA
    Available Since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212155-plasmid-map-sequence-id-420758

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  12. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Promoter
    polyhedrin
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

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  16. pTDpelB-NTwinStrep

    Plasmid
    #45940
    Purpose
    use to create N-terminal PelB-Twin-Strep-tag fusion proteins or dual tagged fusion with N-terminal PelB-Twin-Strep-tag and C-terminal 6x His-tag for periplasmic translocation via PelB signal sequence
    Depositor
    Thorben Dammeyer
    Article
    Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.
    Type
    Empty backbone
    Tags
    6xHis, PelB, and Twin-Strep
    Expression
    Bacterial
    Promoter
    Trc (lactose/IPTG inducible)
    Available Since
    Sept. 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    45940-plasmid-map-sequence-id-64653

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  19. pTriEx-HTNC

    Plasmid
    #13763
    Purpose
    For expression and purification of a His-TAT-NLS-Cre fusion; HIV-TAT promotes cellular uptake of recombinant Cre into mammalian cells.
    Depositor
    Klaus Rajewsky
    Article
    Peitz et al Proc Natl Acad Sci U S A. 2002 Apr 2. 99
    Insert
    His-TAT-NLS-Cre
    Use
    Baculovirus
    Tags
    His Tag, NLS, and TAT
    Expression
    Bacterial
    Promoter
    p10 promoter
    Available Since
    April 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13763-plasmid-map-sequence-id-364424

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Showing: 7641 - 7660 of 33230 results