Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 23,030 results for: his

Clear keywords
Showing: 7641 - 7660 of 23030 results

  1. 4Z0O

    Plasmid
    #69954
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ZCWPW2:JMC01B-H12:C203050 (ZCWPW2 Human)
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7-lacO
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. 4YVD

    Plasmid
    #69960
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLRG1:JMC102-C09:C233452 (PLRG1 Human)
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Promoter
    polyhedrin
    Available Since
    Oct. 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. 4MYI

    Plasmid
    #60103
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PVX_084705:MAC04G-C11:C228845
    Use
    Baculovirus expression
    Tags
    6x His and TEV cleavage site
    Mutation
    See comments
    Promoter
    polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. 4IIM

    Plasmid
    #51325
    Depositor
    Cheryl Arrowsmith
    Insert
    4IIM (ITSN1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. 4IIO

    Plasmid
    #51326
    Depositor
    Cheryl Arrowsmith
    Insert
    4IIO (ITSN2 4IIO, Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 24, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. PTPRJ

    Plasmid
    #38888
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRJ (PTPRJ Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C1239S inactivating mutation
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. CDY

    Plasmid
    #25507
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CDY (CDY2B Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    catalytic domain
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. SGF29 (3MEU)

    Plasmid
    #25317
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SGF29_6 (SGF29 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 115-293
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. SGF29 (3LX7)

    Plasmid
    #25314
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SGF29_3 (SGF29 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids 138-291
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. C6orf197

    Plasmid
    #25258
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    C6orf197:M178-R505 (TBC1D22B Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids M178-R505
    Available Since
    Aug. 13, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. PLXND1 (3H6N)

    Plasmid
    #25187
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PLXND1:A1553-D1656 (PLXND1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids A1553-D1656
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. PP-CDPK1 (3DXN)

    Plasmid
    #25129
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    541.m00134:MAC02T-C05:C40091
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids L72-Q339
    Available Since
    Aug. 5, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pCJ189

    Plasmid
    #162666
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162666-plasmid-map-sequence-id-321465

    Enlarge

  14. pCJ190

    Plasmid
    #162667
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162667-plasmid-map-sequence-id-321443

    Enlarge

  15. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

    Enlarge

  16. pCJ202

    Plasmid
    #162675
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    W159C, S238C; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162675-plasmid-map-sequence-id-321470

    Enlarge

  17. pOPINB-HOIL-1 helix-RBR (233–510)

    Plasmid
    #193859
    Purpose
    Expression of human His-tagged HOIL-1 (RBCK1) helix-RBR (aa 233–510) codon optimized for E. coli
    Depositor
    Bernhard Lechtenberg
    Article
    Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:
    Insert
    HOIL-1 (RBCK1 Human)
    Tags
    3C protease cleavage site and 6x-His tag
    Expression
    Bacterial
    Mutation
    Residues 233–510, codon optimised for expression …
    Promoter
    T7
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    193859-plasmid-map-sequence-id-379616

    Enlarge

  18. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available Since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

    Enlarge

  19. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

    Enlarge

  20. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162684-plasmid-map-sequence-id-321526

    Enlarge

Showing: 7641 - 7660 of 23030 results