We narrowed to 16,623 results for: puromycin

1. PKD1 gRNA (BRDN0001149544)

   Plasmid

   #76841

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PKD1

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PKD1 (PKD1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLX307-VHL(Y98H)-3F-BirA

   Plasmid

   #220148

   Purpose

   Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

   Depositor

   William Sellers

   Article

   Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

   Insert

   von Hippel-Lindau tumor suppressor (VHL Human)
   

   Use

   Lentiviral

   Tags

   3F-BirA

   Expression

   Mammalian

   Mutation

   changed tyrosine 98 to histidine; codon optimized

   Promoter

   EF-1a

   Available Since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2530

   Plasmid

   #144035

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   SOX8 (SOX8 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TFORF2138

   Plasmid

   #143571

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   TRPS1 (TRPS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-mir-17-92

   Plasmid

   #64100

   Purpose

   expresses mir-17-92

   Depositor

   Lin He

   Article

   Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

   Insert

   miR-17-92 (MIR17HG Human)
   

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1P CISD1_1

   Plasmid

   #160773

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-N-Puro-mNeon-ACTB

   Plasmid

   #207752

   Purpose

   Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   ACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-Tet-On-ARNTL-shRNA2

   Plasmid

   #166914

   Purpose

   Lentivirus for inducible knockdown of human ARNTL(BMAL1)

   Depositor

   Roland Friedel

   Article

   Halawani et al Nat Commun. 2023 Aug 24;14(1):5165. doi:

   Insert

   ARNTL shRNA (BMAL1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1P CISD1_2

   Plasmid

   #160774

   Purpose

   Suppress CISD1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCISD1_2
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO-puro-tetON-shRFP

    Plasmid

    #110940

    Purpose

    Tet-inducible shRNA targeting RFP

    Depositor

    William Hahn

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

    Insert

    shRFP
    

    Use

    Lentiviral and RNAi

    Promoter

    H1/TO

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-Cdt1-mCherry-Puro

    Plasmid

    #193763

    Purpose

    Doxycyline-inducible (TetOn) human Cdt1 (C-terminal mCherry tag), expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    Cdt1-mCherry (CDT1 Human)
    

    Use

    Lentiviral

    Tags

    WT

    Mutation

    human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

    Promoter

    TRE

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid

    #155051

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TFORF1243

    Plasmid

    #143776

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SFPQ (SFPQ Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TFORF1464

    Plasmid

    #144104

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    VAV1 (VAV1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

    Plasmid

    #112837

    Purpose

    transient/stable overexpression

    Depositor

    Tomasz Prószyński

    Article

    Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

    Insert

    Grb2 (Grb2 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SH2 + SH3 (C-term) domains of Grb2

    Available Since

    Aug. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK-IKZF3-NTERM-GFP

    Plasmid

    #185774

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, IKZF3 minidegron

    Mutation

    none

    Promoter

    PGK

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2137

    Plasmid

    #143570

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TRPS1 (TRPS1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid

    #155053

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TNIK gRNA (BRDN0001146045)

    Plasmid

    #75849

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human TNIK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    TNIK (TNIK Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SFFV-IKZF3-NTERM-GFP

    Plasmid

    #185764

    Purpose

    Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

    Depositor

    Alessandra Ianari, William Sellers

    Article

    Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    V5, IKZF3 minidegron

    Mutation

    none

    Promoter

    SFFV

    Available Since

    Sept. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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