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pHAGE-AKT3-S370P

Plasmid

#116106

Purpose

Lentiviral expression of AKT3 S370P

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Mutation

S370P

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600L

Plasmid

#116207

Purpose

Lentiviral expression of BRAF V600L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600L

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-V600Ifs*13

Plasmid

#116198

Purpose

Lentiviral expression of BRAF V600Ifs*13

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600Ifs*13

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL1

Plasmid

#115443

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL1 (MBNL1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLX317-MBNL2

Plasmid

#115444

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

MBNL2 (MBNL2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NFIA+SOX9

Plasmid

#129455

Purpose

Inducible overexpression of NFIA and Sox9 in human cells.

Depositor

Su-Chun Zhang

Article

Li et al Stem Cell Reports. 2018 Oct 9;11(4):998-

Insert

Sox 9 (SOX9 Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A3

Plasmid

#189750

Purpose

The construct was used to express recombinant ALDH1A3 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-ALDH1A1

Plasmid

#189749

Purpose

The construct was used to express recombinant ALDH1A1 in E.Coli. The purified protein was used for activity assay.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A1 (ALDH1A1 Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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NONO2_IDR-EGFP

Plasmid

#232758

Purpose

Expresses NONO2_IDR-EGFP

Depositor

Isaac Hilton

Article

Li et al Nucleic Acids Res. 2025 Feb 8;53(4):gkaf

Insert

NONO2_IDR-EGFP (NONO Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD

Plasmid

#216248

Purpose

HDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSD

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human RANGAP1 (RANGAP1 Human)

Use

CRISPR and TALEN; Hdr template

Tags

miniIAA7.3xFlag.P2A.BSD

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A3-Neo

Plasmid

#189744

Purpose

The construct was used to express GFP tagged human ALDH1A3 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A3 (ALDH1A3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 WT

Plasmid

#121486

Purpose

2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-EGFP-ALDH1A2-Neo

Plasmid

#189743

Purpose

The construct was used to express GFP tagged human ALDH1A2 in mammalian cells for the analysis of the subcellular location of this protein.

Depositor

Robert Sobol

Article

Li et al Commun Biol. 2021 Dec 21;4(1):1420. doi:

Insert

ALDH1A2 (ALDH1A2 Human)

Tags

EGFP

Expression

Mammalian

Promoter

PGK

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-CELF4

Plasmid

#115445

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

CELF4 (CELF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-SEC61B.N-BSD.P2A.miniIAA7.3xFlag

Plasmid

#216250

Purpose

HDR template to tag endogenous human SEC61B N-terminus with BSD.P2A.miniIAA7.3xFlag

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

HDR template for human SEC61B (SEC61B HDR template, Human, Synthetic)

Use

CRISPR; Hdr template

Tags

BSD.P2A.miniIAA7.3xFlag

Expression

Mammalian

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMal-Abl-PRM 5R

Plasmid

#112088

Purpose

Bacterial expression plasmid containing His and MBP tags for 5 PRM motif repeats of Human Abl.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-5R (ABL1 Human)

Tags

His-6 and MBP

Expression

Bacterial

Mutation

construct contains only the PRM motif of Abl1

Promoter

Lac

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#216251

Purpose

Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

Cas9 and sgSEC61B.N1 (SEC61B Human)

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(PX-LD)

Plasmid

#214422

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)

Use

Lentiviral

Tags

CFAST-Halo and FKBP-V5-RspAN

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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