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GreenT-EC.b - Display - CyRFP1

Plasmid

#193944

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.b

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 May 28;285(22):16562-7

Insert

Timeless (TIMELESS Human)

Tags

Flag

Expression

Insect

Available Since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
GreenT-EC.c - Display - CyRFP1

Plasmid

#193945

Purpose

Mammalian expression vector for a low affinity calcium indicator exposed to the cell surface

Depositor

Oliver Griesbeck

Article

Valiente-Gabioud et al Nat Commun. 2023 Oct 5;14(1):6220. doi:

Insert

GreenT-EC.c

Tags

Igk_secretion - HA-Flag and PDGFR

Expression

Mammalian

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMT-Hrp48-ADARcd-E488Q-V5

Plasmid

#139685

Purpose

Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

Depositor

Michael Rosbash

Article

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Hrp48, ADAR (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-sfGFP-3xP3-RFP

Plasmid

#127566

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

sfGFP

Use

CRISPR

Expression

Insect

Available Since

July 13, 2019

Availability

Academic Institutions and Nonprofits only

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Brp-T2A-QF2w-Hsp70-3XP3-dsRed

Plasmid

#141094

Purpose

Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

Depositor

Carolyn McBride

Article

Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

Insert

bruchpilot

Use

CRISPR

Expression

Insect

Mutation

coding sequence downstream of CRISPR cut site, sy…

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Tags

tdTomato

Expression

Insect

Promoter

QUAS

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pB[U6-3 wex2 PUb-DsRed]

Plasmid

#169010

Purpose

For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

white ex2 sgRNA

Use

Piggybac

Expression

Insect

Promoter

Drosophila melanogaster U6:3

Available Since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJFRC-nSyb-IVS-PhiC31

Plasmid

#133868

Purpose

Replace the Nsyb promoter to express PhiC31 under the control of any promoter of interest.

Depositor

Tom Clandinin

Article

Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

Insert

PhiC31 Recombinase (int )

Expression

Insect

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

Plasmid

#63167

Purpose

GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

Insert

"spaghetti monster GFP-cMyc"

Tags

N-myristoylation

Expression

Insect

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-Rab32

Plasmid

#31744

Depositor

Ron Vale

Article

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab32 (Rab32 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available Since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pTSAR3.5t

Plasmid

#210257

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

eBFP2
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTSAR3.4t

Plasmid

#210256

Purpose

Fluorescent reporter for Type III secretion system activity in Shigella flexneri

Depositor

François-Xavier Campbell-Valois

Article

Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

Inserts

mCherry
superfolder GFP

Tags

ssra

Expression

Bacterial

Promoter

ipaH7.8p and rpsM

Available Since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pTS2311-Tier1-spacer-PPGK-NLuc_TB

Plasmid

#169552

Purpose

Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 preluded by a 5' spacer sequence (spacer-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-NanoLuc Luciferase - mTagBFP fusion

Expression

Mammalian

Promoter

PPGK

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac_HT_B_6xHis-TEV-GFP-NBR1

Plasmid

#190928

Purpose

Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

Depositor

Sascha Martens

Article

Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

Insert

NBR1 (NBR1 Human)

Tags

Strep-TEV-GFP

Expression

Insect

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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10XSTAT92E–luciferase

Plasmid

#37393

Depositor

Norbert Perrimon

Article

Baeg et al Genes Dev. 2005 Aug 15;19(16):1861-70. E

Insert

SOCS36E enhancer fragment (Socs36E Fly)

Use

Luciferase; Jak/stat reporter construct

Expression

Insect

Promoter

Hsp70

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pLV_Tre3g_CalcireticulinSS_mTurquoise_D4-cameleon_REAch2_KDEL

Plasmid

#216253

Purpose

FRET-based calcium sensor located in the ER lumen, Tet-inducible

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

ER-targeted D4 second generation cameleon (FRET calcium sensor)

Use

Lentiviral

Tags

REAch and mTurquoise

Promoter

TRE3G

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTattB_NSlmb-vhhGFP4

Plasmid

#35577

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Insect

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pET21-pelB-GST-4

Plasmid

#228986

Purpose

For bacterial expression of anti-GST nanobody GST-4, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-4

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

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