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pBS-MCS1M(1)Kana-mCMV-TK-pA

Plasmid

#22060

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

TK

Expression

Mammalian

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pOEM-GED-EGFP

Plasmid

#102782

Purpose

Baculovirus rescue vector, VSVGED pseudotype, eGFP

Depositor

Elly Tanaka

Article

Oliveira et al Dev Biol. 2018 Jan 15;433(2):262-275. do

Insert

EGFP

Use

Baculoviral rescue shuttle vector

Expression

Insect and Mammalian

Promoter

CMV

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Puro Empty (w609-1)

Plasmid

#20957

Purpose

3rd gen lentiviral RNAi empty control vector, 3’LTR insertion, Puro

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pUCBB-ptet-eGFP

Plasmid

#32554

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Promoter

ptet

Available Since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (29-339)

Plasmid

#136544

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (29-339) and His-tag

Expression

Bacterial

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR/pTER shLUC (w177-1)

Plasmid

#17472

Purpose

Gateway entry vector for Firefly Luciferase shRNA under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase shRNA

Use

RNAi; Entry vector

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-ACG site)

Plasmid

#170155

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(ACG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAC8-TwinStrep-3C-DrHDAC6 (40-831)

Plasmid

#180226

Purpose

Baculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)

Depositor

Patrick Matthias

Article

Langousis et al Methods Mol Biol. 2023;2589:467-480. doi

Insert

Histone deacetylase 6 (hdac6 Zebrafish)

Tags

TwinStrep-3C

Expression

Insect

Promoter

polyhedrin

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-T7-hAIM2

Plasmid

#51541

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GFP and T7

Expression

Mammalian

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmGW182

Plasmid

#22419

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

gawky, variant A (gw Fly)

Use

Drosophila expression

Tags

EGFP

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Tags

6XHis

Expression

Bacterial

Promoter

T7

Available Since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pGAN101

Plasmid

#131123

Purpose

Plasmid for overexpression of prion domains to induce prions

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Type

Empty backbone

Tags

mKate2

Expression

Yeast

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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HA Raptor mutant 9

Plasmid

#8476

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Mutation

1191RVYDRR1196-DAAADD (R1191D, V1192A, Y1193A, D1…

Available Since

June 20, 2005

Availability

Academic Institutions and Nonprofits only
JAB2603

Plasmid

#190390

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_5XAmtRO::GFPint-NLS-ADHt

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pILGFPU0 (R)

Plasmid

#83562

Purpose

Express yEGFP-ERG9 ER targeting peptide-CLN2PEST under the control of TEF1 promoter

Depositor

Claudia Vickers

Article

Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

Insert

TEF1 promoter-yEGFP-ERG9 ER targeting peptide-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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rNL1(46-638)

Plasmid

#20172

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neuroligin 1 (Nlgn1 Rat)

Tags

C-terminal 6X His tag

Expression

Insect

Mutation

no splice sites

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pEB2-mRFP1

Plasmid

#104001

Purpose

Plasmid encoding mRFP1

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mRFP1

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-UAG site)

Plasmid

#170138

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(UAG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-EGFP100

Plasmid

#46309

Depositor

Judy Lieberman

Article

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
EGFP100 DNA-1
linker
EGFP100 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available Since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only