We narrowed to 19,051 results for: Tec

1. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

   Plasmid

   #121509

   Purpose

   Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgFah.intron9
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4-HA-Flag-natT1R2 d835

   Plasmid

   #113959

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-834

   Promoter

   cmv

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAVsc.U6-sgIdua.CMV/CB-EGFP

   Plasmid

   #121511

   Purpose

   Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgIdua
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAVsc.U6-sgAspa.CMV/CB-EGFP

   Plasmid

   #121510

   Purpose

   Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa).

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   sgAspa
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOCC176

   Plasmid

   #118891

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP, cleavable with TEV

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-SNAP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; SNAP, cleav…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO-TPI_PTC160-xrRNA-4H

   Plasmid

   #108378

   Purpose

   FRT integration of TPI reporter with premature termination codon (PTC160); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   PTC160 TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO-TPI_WT-xrRNA-4H

   Plasmid

   #108377

   Purpose

   FRT integration of wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b+C3(R112C/T180C)X

   Plasmid

   #108196

   Purpose

   Expresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

   Insert

   P450cam-PCNA3(R112C/T180C)-PdX fusion protein
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C/T180C(PCNA3); C73S/C85S (PdX)

   Promoter

   T7 promoter

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSTAP-seq_fly-zfh1_spikeIn-1

   Plasmid

   #86387

   Purpose

   Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster zfh1 enhancer & D. pseudoobscura eve core promoter
   

   Use

   Stap-seq spike in vector

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PatoK-OG241

    Plasmid

    #72840

    Purpose

    NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. OG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoK
    

    Expression

    Bacterial

    Promoter

    PatoK

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. PatoL-LOG241

    Plasmid

    #72846

    Purpose

    Induced by acetoacetate to express GFP using atoSC two-component system in E. coli. Contains the atoDAEB promoter (Pato) with a strong RBS, Bba_B0034 (iGEM). LOG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoL
    

    Expression

    Bacterial

    Promoter

    PatoL

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. PatoK-LOG241

    Plasmid

    #72848

    Purpose

    NOT induced by acetoacetate to express GFP. Contains the native atoDAEB promoter (Pato) from E. coli DH5A, but out-of-frame with GFP gene. LOG241 backbone.

    Depositor

    Chris Barnes

    Article

    Rutter et al Biotechnol Bioeng. 2021 Nov;118(11):4278

    Insert

    PatoK
    

    Expression

    Bacterial

    Promoter

    PatoK

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. His8:MBP-tev-Ala-eK-Flv

    Plasmid

    #83393

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. His8:MBP-tev-Ile-eK-Flv

    Plasmid

    #83392

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. His8:MBP-tev-Tyr-eK-Flv

    Plasmid

    #83391

    Purpose

    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

    Depositor

    Nico Dissmeyer

    Article

    Mot et al New Phytol. 2017 Mar 9. doi: 10.1111/nph

    Insert

    flavin binding protein MioC
    

    Use

    Synthetic Biology

    Tags

    3xHA, 8xHis, and MBP

    Expression

    Bacterial

    Available Since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSTAP-seq_fly-zfh1_spikeIn-3

    Plasmid

    #86389

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#1
    

    Use

    Stap-seq spike in vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSTAP-seq_fly-zfh1_spikeIn-4

    Plasmid

    #86390

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura ush core promoter -TSS#2
    

    Use

    Stap-seq spike in vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSTAP-seq_fly-zfh1_spikeIn-2

    Plasmid

    #86388

    Purpose

    Expression vector for a transcript origination from a D. pse core promoter, which is used as trasfection control (normalization), i.e. STAP-seq screening vector containing one specific core promoter

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Insert

    D. melanogaster zfh1 enhancer & D. pseudoobscura CG32369 core promoter
    

    Use

    Stap-seq spike in vector

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCellFree_G03 AKT1

    Plasmid

    #67102

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    AKT1 (AKT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 BMPR1A

    Plasmid

    #67105

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BMPR1A (BMPR1A Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only