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pEPQDKN0729

Plasmid

#162316

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pAL198

Plasmid

#22281

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

p!SV40-mCherry-WaspGBD

Use

Synthetic Biology

Expression

Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pAEM7

Plasmid

#136422

Purpose

salL gene from S. tropica CNB-440 ligated into pHIS8

Depositor

Bradley Moore

Article

Eustaquio et al Nat Chem Biol. 2008 Jan;4(1):69-74. doi:

Insert

salL

Tags

His8 tag

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pICH42211

Plasmid

#50255

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL50006

Plasmid

#50319

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (Photinus pyralis)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL30005

Plasmid

#50299

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

N terminal FLAG tag (3x FLAG octapeptide)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSUPER ERbeta shRNA

Plasmid

#35561

Depositor

Harish Srinivas

Article

Zhang et al Mol Endocrinol. 2009 Feb;23(2):146-56. E

Insert

ERbeta

Use

Retroviral

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-SYNZIP20

Plasmid

#80674

Purpose

Encodes the synthetic coiled coil peptide SYNZIP20

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP20

Use

Gateway Cloning

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-miR-31 sponge

Plasmid

#25025

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

CMV-miR-31 sponge

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
pICSL50011

Plasmid

#50317

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, EOS photoactivatable fluorescent protein (L. hemprichii)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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