We narrowed to 7,771 results for: Pol
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Plasmid#257189PurposeExpression construct for TfCut2_ICCG_b0.45_h80_dd90_12 gene, codon optimized for expression in E. coliDepositorInsertTfCut2_ICCG_b0.45_h80_dd90_12
ExpressionBacterialMutationR18T, S19R, S23A, V24T, S25A, E26Q, E27T, N28T, R…Available sinceJuly 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
TfCut2_ICCG_b0.45_h80_dd90_02
Plasmid#257186PurposeExpression construct for TfCut2_ICCG_b0.45_h80_dd90_02 gene, codon optimized for expression in E. coliDepositorInsertTfCut2_ICCG_b0.45_h80_dd90_02
ExpressionBacterialMutationR18T, S19R, E27T, N28S, A34V, R46T, E47T, N48T, N…Available sinceJuly 29, 2026AvailabilityAcademic Institutions and Nonprofits only -
Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)
Plasmid#217797PurposeVariant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.DepositorInsertCdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationCdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-D842V+R914W_EGFP-PEST_reporter
Plasmid#172599PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-D842V+R914W mutant signaling.DepositorInsertmyc-PDGFRA-D842V+R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-D842V+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRA-K627R+R914W_EGFP-PEST_reporter
Plasmid#172600PurposeMammalian fluorescent (EGFP-PEST) reporter plasmid for PDGFRA-K627R+R914W mutant signaling.DepositorInsertmyc-PDGFRA-K627R+R914W (PDGFRA Human, Synthetic)
UseEgfp-pest_reporterTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPDGFRA-K627R+R914WPromoterCMVAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DED/AAA 535-888 USP7
Plasmid#131255PurposeMammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationA mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-N-Myc_DW164AA + DED/AAA USP7
Plasmid#131256PurposeMammalian expression of N-terminally Myc-tagged USP7, with mutations in the TRAF-like domain (DW164AA) and UBL2 (DE758AA_D764A)DepositorInsertUbiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
TagsMycExpressionMammalianMutationContains point mutations that convert USP7 aspart…Available sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)
Plasmid#91820PurposeYeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTDDepositorInsertRPO21 (RPO21 Budding Yeast)
Tags10xHisExpressionYeastMutation5xGly linker, PreScission Protease site (LEVLFQGP…PromoterEndogenous Rpb1 promoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
RLC-ELP-ybbR-LA-GB1-GB1-Xmod-DocIII-Ctag
Plasmid#257334PurposePolyprotein for applying force to the N- and C-termini of the lever arm in the RLC complex of human β-cardiac myosinDepositorTagsCtag, Dockerin III, ELP120 (Elastin-Like Polypept…ExpressionBacterialMutationonly amino acids # 810-846 are usedPromoterT7Available sinceJuly 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
LA-ELP-ybbR-RLC-ddfln4-Xmod-DocIII-Ctag
Plasmid#257335PurposePolyprotein for applying force to the N- and C-termini of the RLC in the RLC complex of human β-cardiac myosinDepositorTagsCtag, Dockerin III, ELP120 (Elastin-Like Polypept…ExpressionMammalianMutationonly amino acids # 810-846 are usedPromoterT7Available sinceJuly 17, 2026AvailabilityAcademic Institutions and Nonprofits only -
GoldenBraid 2.0 E. coli Kit
Plasmid kit#1000000284PurposeA toolkit designed for use in E. Coli and adhering to the GoldenBraid 2.0 standard.DepositorApplicationCloning and Synthetic Biology, Genome EditingVector typeBacterial ExpressionEditing typeCRISPRCloning typeGolden Gate (GoldenBraid)Available sinceSept. 11, 2026AvailabilityAcademic Institutions and Nonprofits only