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pAd-M3cherry
Plasmid#61041PurposeThis plasmid mediates polycistronic expression of 4 genes: Ngn3, Pdx1, Mafa, and mCherry. It can be used to convert mouse pancreatic acinar cells to induced beta-like cells in vivo.DepositorUseAdenoviralTagsExpressionMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-Fast-1 wild-type
Plasmid#16521DepositorInsertFAST-1 (FOXH1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-tdTomato
Plasmid#159275PurposeHuman AAVS1 targeting vector for knockin of a CAGGS promoter-driven tdTomato cassette (cloned between AgeI and PacI). Targeted cells will be puromycin resistant.DepositorInserttdTomato
UseAAV; S1 knockin donor vectorTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
HyWnt3:Luc
Plasmid#192986PurposePlasmid to test the Hydra Wnt3 promoter activityDepositorInsertHydra Wnt3 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTRm
Plasmid#127197PurposepGTRm is the template plasmid for PCR & golden gate assembly driven generation of polycistronic tRNA-gRNA sequencesDepositorInserttRNA-gRNA PCR template
UseTemplate plasmid for generation of golden gate pc…TagsExpressionMutationPromoterNoneAvailable sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Zeo-CnB
Plasmid#191965PurposeExpression of CnB (calcineurin subunit beta).DepositorInsertCalcineurin subunit beta (PPP3CB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
915-Plakophilin 2a-Flag
Plasmid#32230DepositorInsertPlakophilin 2a (PKP2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
p6872 pHAGE-N-V5-MAML1-FL
Plasmid#37048DepositorInsertpHAGE-N-V5-MAML1-FL (MYLIP Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVtAvailable sinceJuly 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Smad3
Plasmid#172098PurposeExpresses fluorescently tagged human Smad3 in mammalian cells.DepositorInsertSmad 3 (Smad3 Mouse)
UseTagsmNeonGreenExpressionMammalianMutationC49S mutation in mCeruleanPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHRSin_hTCRβε
Plasmid#187354PurposeLentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 epsilonDepositorInsertGPa3b17 hTCR beta, and truncated hCD3 epsilon
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
V51 pIRESpuro-GLUE (pGLUE)
Plasmid#15100Purposetandem-affinity purification plasmid; streptavidin-(streptag) and calmodulin-(CBP) binding affinity tagsDepositorTypeEmpty backboneUseTagsCBP tag, HA, and streptagExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHRSin_hTCRβ
Plasmid#187361PurposeLentiviral expression of GPa3b17 hTCR betaDepositorInsertGPa3b17 hTCR beta
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETconNK-TEM1(S70A,D179G)
Plasmid#81166PurposepETconNK with TEM1(S70A,D179G) beta-lactamaseDepositorInsertTEM-1 beta-lactamase
UseTagsc-mycExpressionBacterial and YeastMutationDeleted the sec transport tag. Plasmid encodes H2…PromoterGAL1 and gal1Available sinceFeb. 21, 2017AvailabilityAcademic Institutions and Nonprofits only
