We narrowed to 7,300 results for: RAP

1. pCS2-SP-FKBP-mCherry-TM-V5

   Plasmid

   #186226

   Purpose

   mRNA synthesis of rapalog-induced cell adhesion molecule

   Depositor

   Yoshihiro Morishita

   Article

   Usami et al Genes Cells. 2022 Jun;27(6):436-450. doi

   Insert

   SP-FKBP-mCherry-TM-V5
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD375

   Plasmid

   #91825

   Purpose

   Codon-optimized mKate2 donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   mKate2-C1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMLS234

   Plasmid

   #73682

   Purpose

   SapTrap 3-site Destination vector for N-terminal GFP tagging with embedded Cbr-unc-119 selectable marker

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   GFP + Cbr-unc-119
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD397

   Plasmid

   #91822

   Purpose

   AID FP-slot donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD398

   Plasmid

   #91832

   Purpose

   AID NT-tag linker donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Auxin-inducible degron (AID)
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFOSGE_COF3D10

   Plasmid

   #71004

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   MED23 (MED23 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pXF262

   Plasmid

   #139038

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   cox-4 (no STOP codon) (W09C5.8 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSDH50

   Plasmid

   #139037

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJF7

   Plasmid

   #139036

   Purpose

   MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   tomm-20 (no STOP codon) (tomm-20 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSDH66

    Plasmid

    #139053

    Purpose

    MosSCI SapTrap assembly: Cassette 4 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    unc-54 3'UTR (unc-54 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHW622

    Plasmid

    #229868

    Purpose

    Entry vector with reverse FRT3 (rFRT3) in slot 7 (for SapTrap assembly)

    Depositor

    Han Wang

    Article

    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

    Insert

    reverse FRT3 (rFRT3)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-Myo1-IQ-Tail

    Plasmid

    #187363

    Purpose

    Expresses rat myosin 1b isoform b IQTail domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

    Insert

    myosin 1b isoform b IQTail domain (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXF90

    Plasmid

    #139044

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag::HDEL (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMLS415

    Plasmid

    #114272

    Purpose

    SapTrap donor vector for point mutagenesis; pENTR-221::syntron(lox2272-119+)

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

    Insert

    Cbr-Unc-119
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Mutation

    *please see depositor comment below

    Promoter

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.3 Gag-imCherry

    Plasmid

    #208882

    Purpose

    codes for Gag from HIV-1 with an internal mCherry fluorescent protein

    Depositor

    Raphael Gaudin

    Article

    Diana Brychka et al bioRxiv

    Insert

    HIV-1 Gag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcs2-GFP-IRES-mCherry

    Plasmid

    #226098

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid

    #230996

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLC-237

    Plasmid

    #73191

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    ParaB-relE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ParaB

    Available since

    June 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLC-246

    Plasmid

    #73190

    Purpose

    Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

    Depositor

    Swaine Chen

    Article

    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

    Insert

    PrhaB-tse2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PrhaB

    Available since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMET7-GAG-GW

    Plasmid

    #194634

    Purpose

    Gateway destination vector for mammalian expression of Virotrap bait constructs

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    PQS1

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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