We narrowed to 18,942 results for: Tec

1. Antares-SEPLuc IRESv24

   Plasmid

   #141087

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-IRESv24-SEPLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCML 313

   Plasmid

   #98586

   Purpose

   pNYFP + CsrB-2MS2BD. Expresses NYFP control fusion and RNA-2MS2BD fusion in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Insert

   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCML 314

   Plasmid

   #98584

   Purpose

   pCsrA-NYFP + CsrB-2MS2BD. Expresses protein-NYFP and RNA-2MS2BD fusions in E. coli for TriFC assay (ampR).

   Depositor

   Lydia Contreras

   Article

   Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

   Inserts

   CsrA
   csrB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLacO

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLNHA-C1-Hs4E-BP1

   Plasmid

   #79438

   Purpose

   Expresses lambdaN-HA-tagged human 4E-BP1 in Mammalian cells

   Depositor

   Elisa Izaurralde

   Article

   Peter et al Mol Cell. 2015 Mar 19;57(6):1074-87. doi

   Insert

   Hs4E-BP1 (EIF4EBP1 Human)
   

   Use

   Tags

   lambdaN-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNvT-MHC::mCh

   Plasmid

   #67943

   Purpose

   Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

   Depositor

   Ulrich Technau

   Article

   Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   mCherry
   

   Use

   Transgenic reporter in the sea anemone, nematoste…

   Tags

   Expression

   Mutation

   Promoter

   Myosin Heavy Chain1 (MyHC1)

   Available Since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

   Plasmid

   #216694

   Purpose

   Synchronize trafficking of GPI-AP from the ER (RUCH system)

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Inserts

   FIREmate-KDEL
   SBP-mApple-FIREtag-GPI
   

   Use

   Tags

   FIREtag, IL2 signal sequence, Streptavidin Bindin…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFEndoNA2

   Plasmid

   #174667

   Purpose

   Expresses GFP-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA2 fusion protein
   

   Use

   Tags

   Histag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. FpG5

   Plasmid

   #69443

   Purpose

   lentiviral GFP reporter plasmid for high throughput functional detection of active transcriptional regulatory DNAs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pmCherry-C1-MKLP1

   Plasmid

   #70154

   Purpose

   mammalian expression of MKLP1 with an mCherry fusion

   Depositor

   Masanori Mishima

   Article

   Joseph et al EMBO J. 2012 May 30;31(11):2604-14. doi:

   Insert

   MKLP1 (KIF23 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pExpreS2-1-C3-10H-SIII

    Plasmid

    #175444

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-10His-TwinStrep and BiP Secretion Peptide

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-DsRed2

    Plasmid

    #112219

    Purpose

    Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

    Depositor

    Yinming Liang

    Article

    Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

    Insert

    DsRed2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    DsRed2 contains a silent nucleotide substitution …

    Promoter

    Available Since

    July 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-C1-BBS2

    Plasmid

    #218712

    Purpose

    Expresses N-terminally EGFP-tagged BBS2 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS2 (BBS2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-Flag-hOct4

    Plasmid

    #20072

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    hOct4 (POU5F1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

    Plasmid

    #216696

    Purpose

    Synchronize trafficking of TNFalpha from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    Tumor Necrosis Factor alpha (TNFa)
    

    Use

    Tags

    FIREtag, Streptavidin Binding Protein (SBP), and …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. ZIKV_Sensor_27B_LacZ

    Plasmid

    #75006

    Purpose

    Toehold switch sensor to detect Zika Virus

    Depositor

    James Collins, Alexander Green

    Article

    Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

    Insert

    ZIKV_sensor_27B_LacZ
    

    Use

    Tags

    LacZ

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHLuc (Antares-SEPLuc C1A4E+6)

    Plasmid

    #141089

    Purpose

    final optimized bioluminescent reporter for detecting extracellular pH changes in tumor acidosis

    Depositor

    Jeak Ling Ding

    Article

    Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

    Insert

    Antares-T2A-puromycin-IRESv24-SEP-C1A4E+6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCD86-EGFP

    Plasmid

    #133858

    Purpose

    Mammalian expression of CD86 C-terminally tagged with EGFP

    Depositor

    Dave Piston

    Article

    Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FLAG-VSVG

    Plasmid

    #80606

    Purpose

    Mammalian expression vector that expresses a VSV-G protein with a FLAG tag near the N-terminus of the protein

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    VSV-G
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-BBS7

    Plasmid

    #218715

    Purpose

    Expresses N-terminally EGFP-tagged BBS7 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    BBS7 (BBS7 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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