We narrowed to 874 results for: VILL

1. pLPC-puromycin-binary-MDH1-3xFLAG-ME1- HA

   Plasmid

   #184549

   Purpose

   Retroviral vector to co-express human MDH1 with 3xFLAG tag and human ME1 with HA tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUltra-MDH1-ME1

   Plasmid

   #184465

   Purpose

   Lentiviral vector for tri-cistronic expression of EGFP, MDH1 and ME1 (seperated by P2A and T2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Inserts

   Malate dehydrogenase 1 (MDH1 Human)
   Malic Enzyme 1 (ME1 Human)
   

   Use

   Lentiviral

   Tags

   fused to T2A

   Expression

   Mammalian

   Mutation

   deletion of stop codon

   Promoter

   UbC

   Available since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SARS2SΔC-H2gp41

   Plasmid

   #170389

   Purpose

   Generation of SARS-CoV-2 spike pseudotyped lentivirus

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   SARS-CoV-2 Spike (S SARS-CoV-2)
   

   Use

   Tags

   HIV gp41

   Expression

   Mammalian

   Mutation

   Deletion of C terminal and fusion with H2 from HI…

   Promoter

   CMV

   Available since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pTRIP-SFFV-Hygro-2A-TMRPSS2

   Plasmid

   #170390

   Purpose

   Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

   Depositor

   Nir Hacohen

   Article

   Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

   Insert

   TMPRSS2 (TMPRSS2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV

   Available since

   June 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. FGFR2 3xFlag

   Plasmid

   #110063

   Purpose

   FGFR2 expression in mammalian cells (JCOPCO paper)

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Dec. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FGFR2 F276C 3xFlag

   Plasmid

   #110064

   Purpose

   FGFR2 F276C expression in mammalian cells (JCOPO paper)

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mutated Phenylalanine 276 to Cysteine (F276C)

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. FGFR2 K41E 3xFlag

   Plasmid

   #110105

   Purpose

   expresses FGFR2 with a single amino acid change in mammalian cells

   Depositor

   Martin Fernandez-Zapico

   Article

   Egan et al JCO Precis Oncol. 2017;2017:PO.17.00018.

   Insert

   fibroblast growth factor receptor 2 (FGFR2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   mutated Lysine 41 to Glutamic acid (K41E)

   Promoter

   CMV

   Available since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKAR2-Br512g3.1

   Plasmid

   #171200

   Purpose

   Expresses Br512g3.1 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Use

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKAR2-Br512g3.2

   Plasmid

   #171201

   Purpose

   Expresses Br512g3.2 polymerase in E.coli

   Depositor

   Andrew Ellington

   Article

   Bhadra et al medRxiv 2022.08.09.22278605

   Insert

   Engineered Bst DNA Polymerase I Large Fragment
   

   Use

   Tags

   8x His tag; Villin Headpiece 47 amino acid (vHP47…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSK9065

    Plasmid

    #84345

    Purpose

    tightly controlled expression of mRFP fusion proteins in S. aureus

    Depositor

    Neville Firth

    Article

    Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

    Type

    Empty backbone

    Use

    Tags

    mRFPmars

    Expression

    Bacterial

    Mutation

    Promoter

    Pxyl/tet

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKAR2-Br512

    Plasmid

    #161875

    Purpose

    Expresses Br512 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Maranhao et al medRxiv

    Insert

    Modified DNA Polymerase I Large Fragment (Br512)
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7, tetPA

    Available since

    Nov. 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pTol-Promoter:NLS-tdMCP-eGFP

    Plasmid

    #86248

    Purpose

    For generating transgenic lines that ubiquitously expressing the fluorescent MCP reporter

    Depositor

    Florence Marlow

    Article

    Campbell et al Development. 2015 Apr 1;142(7):1368-74.

    Insert

    beta Actin promoter:NLS-tdMCPeGFP
    

    Use

    Used with transposase to make transient or stable…

    Tags

    NLS-tdMCP-eGFP

    Expression

    Mutation

    Promoter

    Beta Actin

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOT_7-lenti-EFS-SS1-BBz-2A-puro

    Plasmid

    #217990

    Purpose

    Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Xue et al bioRxiv 2024.11.27.625752

    Insert

    SS1-BBz CAR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOPTXLUC

    Plasmid

    #68504

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    OPTX

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiFUCCI(CA)5

    Plasmid

    #223176

    Purpose

    Lentiviral fluorescent ubiquitination-based cell cycle indicator (FUCCI)

    Depositor

    Neville Sanjana

    Article

    Liang et al Cell. 2024 Dec 26;187(26):7637-7654.e29.

    Insert

    FUCCI(CA)5
    

    Use

    Lentiviral

    Tags

    AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKAR2-Br512_Mut235

    Plasmid

    #179278

    Purpose

    Expresses Br512_Mut235 polymerase in E.coli

    Depositor

    Andrew Ellington

    Article

    Paik et al Biochemistry. 2021 Nov 11. doi: 10.1021/

    Insert

    Engineered Bst DNA Polymerase I Large Fragment
    

    Use

    Tags

    8x His tag; Villin Headpiece 47 amino acid (HP47)…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiCCAT1-Red

    Plasmid

    #226521

    Purpose

    CCAT1 transgene with fluorescent protein mKate2

    Depositor

    Neville Sanjana

    Article

    Caragine et al Nat Commun. 2025 Feb 13;16(1):1611. doi:

    Insert

    EF1a_mKate2_CMV_CCAT1 (CCAT1 Human)
    

    Use

    Lentiviral

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiRNAGuide_001 - hU6-RfxCas13d-DR1-BsmBI-EFS-Puro-WPRE

    Plasmid

    #138150

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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