We narrowed to 2,677 results for: calcium

1. Mouse Alpha2 delta-2 pMT2

   Plasmid

   #58732

   Purpose

   expression of mouse Alpha2 delta-2 in mammalian cells

   Depositor

   Annette Dolphin

   Article

   Barclay et al J Neurosci. 2001 Aug 15;21(16):6095-104.

   Insert

   alpha2 delta-2 (Cacna2d2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-syn-FLEX-axon-jYCaMP1s

   Plasmid

   #135421

   Purpose

   Axon-targeted yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Tags

   GAP43 axon targeting sequence

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-syn-FLEX-jYCaMP1s

   Plasmid

   #135420

   Purpose

   Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

   Depositor

   Kaspar Podgorski

   Article

   Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

   Has Service

   AAV1

   Insert

   jYCaMP1s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP Cav2.2 HA D122A pcDNA3

   Plasmid

   #206120

   Purpose

   expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D122A

   Promoter

   CMV

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cav2.2 HA E1658A (EIVA) pcDNA3

   Plasmid

   #206114

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658A mutation in the P loop of Domain IV which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658A

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cav2.1 EIVA (E1707A) pcDNA3

   Plasmid

   #206125

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain IV to to abolish divalent cation binding

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1707A

   Promoter

   CMV

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Cav2.1 HA EIA (E320A) pcDNA3

   Plasmid

   #206124

   Purpose

   expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and a mutation in the P loop of Domain I to abolish divalent cation binding

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1a (Cacna1a Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E320A

   Promoter

   CMV

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBScMXT + Slo1

   Plasmid

   #16194

   Depositor

   Larry Salkoff

   Article

   McCobb et al Am J Physiol. 1995 Sep . 269(3 Pt 2):H76

   Insert

   calcium-activated potassium channel (KCNMA1, Kcnma1 Mouse, Human)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cav2.2 HA EIVA (E1658A) pCAGGS

   Plasmid

   #206082

   Purpose

   expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain IV which reduces trafficking to the cell surface

   Depositor

   Annette Dolphin

   Article

   Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E1658A

   Promoter

   CMV/B-actin

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Cav2.2 HA EIA (E314A) pCAGGS

    Plasmid

    #206081

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation in the P loop of Domain I which reduces trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E314A

    Promoter

    CMV/B-actin

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Cav2.2 HA EIVK (E1658K) pcDNA3

    Plasmid

    #206066

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV

    Available since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Cav2.2 HA EIVK (E1658K) pCAGGS

    Plasmid

    #206096

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and an E1658K mutation in the P loop of Domain IV which abolishes trafficking to the cell surface

    Depositor

    Annette Dolphin

    Article

    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E1658K

    Promoter

    CMV/B-actin

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TRE-mCherry

    Plasmid

    #92202

    Purpose

    Fluorescent protein reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ER-GCaMP6f

    Plasmid

    #182548

    Purpose

    Expresses GCaMP6f on the cytosolic side of the ER (P-450site)

    Depositor

    Chris Richards

    Article

    Aryal et al Anal Chem. 2022 Jan 21. doi: 10.1021/acs

    Insert

    ER localized P450
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PH-Akt-GFP

    Plasmid

    #51465

    Purpose

    Is a biosensor for PIP3/PI(3,4)P2

    Depositor

    Tamas Balla

    Article

    Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

    Insert

    AKT (aa 1–164)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDest-mCherry-N1

    Plasmid

    #31907

    Depositor

    Robin Shaw

    Article

    Hong et al PLoS Biol. 2010 Feb . 8(2):e1000312.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCITF-KCC2

    Plasmid

    #61404

    Purpose

    Mammalian expression vector for human KCC2 under CAG promoter. Coexpression of TdTomato with IRES sequence

    Depositor

    Franck Polleux

    Article

    Bortone et al Neuron. 2009 Apr 16;62(1):53-71. doi: 10

    Insert

    KCC2 (SLC12A5 Human)
    

    Use

    Tags

    IRES-tdTomato-Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. TRE:GFP

    Plasmid

    #163036

    Purpose

    FLiCRE reporter gene for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TRE-ChrimsonR-mCherry

    Plasmid

    #92207

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    ChimsonR-mCherry
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    June 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 10TALE_Pmin_fLuc

    Plasmid

    #160663

    Purpose

    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain

    Depositor

    Roman Jerala

    Article

    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20

    Insert

    10TALE_Pmin_fLuc
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge