We narrowed to 953 results for: dcas9, grna

1. pk335-CARGO-4mer-36kb-USF

   Plasmid

   #227463

   Purpose

   4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 36kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pk335-CARGO-3mer-36kb-USF

   Plasmid

   #227464

   Purpose

   3-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 36kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pk335-CARGO-6mer-33kb-USF

   Plasmid

   #227465

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 33kb Upstream Fgf5
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pk335-CARGO-6mer-26kb-USP

   Plasmid

   #227444

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 26kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pk335-CARGO-6mer-24kb-USP

   Plasmid

   #227445

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 24kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pk335-CARGO-6mer-20kb-USP

   Plasmid

   #227446

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 20kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pk335-CARGO-6mer-11kb-USP

   Plasmid

   #227447

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 11kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pk335-CARGO-6mer-7.5kb-USP

   Plasmid

   #227448

   Purpose

   6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

   Depositor

   Joanna Wysocka

   Article

   Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

   Insert

   CARGO to 7.5kb Upstream Prdm8
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

   Plasmid

   #234371

   Purpose

   T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

   Depositor

   Michael Smanski

   Article

   Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

   Inserts

   NbGP41-sfGFP-VP64-GB1
   Luciferase
   dCas9-13XGP41
   

   Use

   Synthetic Biology

   Tags

   GB1, GP41, and sfGFP

   Expression

   Plant

   Mutation

   Promoter

   Arabidopsis Ubi10 and MTAP1 (synthetic)

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLG1-puro-sgATL1-2

    Plasmid

    #109006

    Purpose

    CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL1-sgRNA #2 (ATL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6 promoter

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX856

    Plasmid

    #62888

    Purpose

    C-term SpCas9 piece of inducible transcriptional activator (dCas9(C)-FKBP-2xNLS-VP64)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa536-1368)
    

    Use

    CRISPR

    Tags

    FKBP12, NLS, NLS SV40, and VP64

    Expression

    Mammalian

    Mutation

    Asparagine 863 to Alanine (N863A)

    Promoter

    CBh

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMM704

    Plasmid

    #68891

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMM733

    Plasmid

    #68898

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL4), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMM764

    Plasmid

    #68901

    Purpose

    IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    L51P

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAbaMCi-GFP-gfp_pJMP2768

    Plasmid

    #208889

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMM710

    Plasmid

    #68893

    Purpose

    IPTG-inducible CRISPRi vector targeting nonsense sequence (NS), pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM731

    Plasmid

    #68896

    Purpose

    IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMM705

    Plasmid

    #68892

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge