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pcDNA3.1/hpa5
Plasmid#59972Purposeintegrin a5 wild-type headpiece expression (a5 beta-propeller and a5 thigh domain)DepositorInserthuman integrin a5 headpiece (ITGA5 Human)
UseTagsacid coiled-coil and StrepII tagExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-hINO80C (1224-1556)
Plasmid#29445DepositorInsertINO80 (INO80 Human)
UseTagsFlagExpressionMammalianMutationINO80 1224-1556 (a.a.)PromoterAvailable sinceAug. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLT3-D835N-V5/HIS
Plasmid#236008Purposeexpression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid LeukemiaDepositorInserthuman FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
UseTagsV5/HisExpressionMammalianMutationD835N substitutionPromoterCMVAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-flag-hKDM4A-polyA
Plasmid#101051Purposein vitro transcription (T7 promoter)DepositorInsertKDM4A (KDM4A Human)
UseTagsFlagExpressionMammalianMutationPromoterT7Available sinceNov. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-SARS-CoV-2-S-RBD-Fc
Plasmid#141183PurposeMammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-FcDepositorInsertSecreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
UseTagsFc region of IgG1 (a.a. Asp-221 to Lys-447) and S…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Ikkb-FLAG(K44A)
Plasmid#23299DepositorInsertIkkbeta (IKBKB Human)
UseTagsFlagExpressionMammalianMutationK44APromoterAvailable sinceApril 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-Cul4A-deltaROC
Plasmid#20713DepositorInsertCullin 4A (CUL4A Human)
UseTagsHA2ExpressionMammalianMutationDeletion of residues 594 to 612 from N-terminusPromoterAvailable sinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2sh-delta458-500
Plasmid#97074PurposeExpresses mutant CARD14/CARMA2 in mammalian cellsDepositorInsertCARD14 isoform 3 (CARD14 Human)
UseTagsHAExpressionMammalianMutationdeleted amino acids 458-500PromoterCMVAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)
Plasmid#238007Purpose300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.DepositorUseRna donor to study rt-dsbrTagsExpressionMammalianMutation3bp insertion signature (ATC) at Cas9 cut site in…PromoterCMVAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1645/1646 RR/EE)
Plasmid#61359Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (1396/1397 SY/WW)
Plasmid#61363Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_GFP_hTLN2-FL
Plasmid#164840PurposeExpression of GFP tagged FL human talin2DepositorInsertTLN2 (TLN2 Human)
UseTagsHis10-GFPExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_NEPTUNE_hTLN2-FL
Plasmid#164842PurposeExpression of NEPTUNE tagged FL human talin2DepositorInsertTLN2 (TLN2 Human)
UseTagsHis10-NEPTUNEExpressionMammalianMutationPromoterAvailable sinceApril 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm5-mCer
Plasmid#211393Purposemammalian expression of human Mcm5 C-terminally fused to mCeruleanDepositorInsertMcm5 (MCM5 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm2-mVen
Plasmid#211398Purposemammalian expression of human Mcm2 C-terminally fused to mVenusDepositorInsertMcm2 (MCM2 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-hMYC
Plasmid#185373PurposeFor mammalian expression of human MYCDepositorInsertMYC (MYC Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hSyncytin-1(deltaFP)
Plasmid#182443PurposehSyncytin-1, fusion peptide deletion(320-340)DepositorInsertERVW-1 (ERVW-1 Human)
UseTagsExpressionMammalianMutationno fusion peptide (320-340)PromoterCMV-FAvailable sinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Mcm2-mCer
Plasmid#211397Purposemammalian expression of human Mcm2 C-terminally fused to mCeruleanDepositorInsertMcm2 (MCM2 Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Cdc45-mVen
Plasmid#211390Purposemammalian expression of human Cdc45 C-terminally fused to mVenusDepositorInsertCdc45 (CDC45 Human)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only
