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We narrowed to 2,916 results for: human cdnas

Showing: 761 - 780 of 2916 results

  1. pcDNA3-SARS-CoV-2-S-RBD-Fc

    Plasmid
    #141183
    Purpose
    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with IgG1-Fc
    Depositor
    Erik Procko
    Article
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Insert
    Secreted receptor binding domain (RBD; a.a. 333-529) of S protein from SARS-CoV-2 fused to Fc of IgG1 (S SARS coronavirus 2)
    Use
    Tags
    Fc region of IgG1 (a.a. Asp-221 to Lys-447) and S…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141183-plasmid-map-sequence-id-288058

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  2. pcDNA3.1-FLT3-D835N-V5/HIS

    Plasmid
    #236008
    Purpose
    expression of the D835N kinase defective mutant variant of human FLT3 that is associated with Acute Myeloid Leukemia
    Depositor
    Pavel Krejčí
    Article
    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:
    Insert
    human FLT3-D835N receptor tyrosine kinase, full length (FLT3 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    D835N substitution
    Promoter
    CMV
    Available since
    April 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    236008-plasmid-map-sequence-id-469587

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  7. pcDNA5/FRT/MALAT1[WT] (pAVA3171)

    Plasmid
    #239347
    Purpose
    Expresses full-length, driven by its own promoter MALAT1 with wild-type ENE and an 11-nt insertion (cgctcgacgta).
    Depositor
    Andrei Alexandrov
    Article
    Che et al Nat Commun. 2025 May 22;16(1):4741. doi:
    Insert
    10,843 bp of MALAT1 of genomic locus amplified from genomic DNA of Hela cells (MALAT1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    An 11-nt insertion (cgctcgacgta)
    Promoter
    Available since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    239347-plasmid-map-sequence-id-479138

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  13. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Agnel Sfeir, Simon Powell
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Tags
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  14. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  15. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Synthetic, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  16. pcDNA3-Mcm5-mCer

    Plasmid
    #211393
    Purpose
    mammalian expression of human Mcm5 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm5 (MCM5 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211393-plasmid-map-sequence-id-420429

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  17. pcDNA5-FRT-hMYC

    Plasmid
    #185373
    Purpose
    For mammalian expression of human MYC
    Depositor
    Ayaz Najafov
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    WT
    Promoter
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    185373-plasmid-map-sequence-id-363449

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  18. pcDNA3-Mcm2-mVen

    Plasmid
    #211398
    Purpose
    mammalian expression of human Mcm2 C-terminally fused to mVenus
    Depositor
    Helmut Pospiech
    Insert
    Mcm2 (MCM2 Human)
    Use
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    211398-plasmid-map-sequence-id-419249

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  19. pcDNA3.1-hSyncytin-1(deltaFP)

    Plasmid
    #182443
    Purpose
    hSyncytin-1, fusion peptide deletion(320-340)
    Depositor
    Masaharu Somiya
    Insert
    ERVW-1 (ERVW-1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    no fusion peptide (320-340)
    Promoter
    CMV-F
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    182443-plasmid-map-sequence-id-357706

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  20. pcDNA3-Mcm2-mCer

    Plasmid
    #211397
    Purpose
    mammalian expression of human Mcm2 C-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm2 (MCM2 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    211397-plasmid-map-sequence-id-420430

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Showing: 761 - 780 of 2916 results