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KTK_346

Plasmid

#180549

Purpose

Entry vector containing gfasPurple

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

gfasPurple

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pQmod3S-GG

Plasmid

#191349

Purpose

Clostridium expression vector (pCB102 origin, specR) with drop-out Plac-RFP cassette flanked with BsaI sites

Depositor

Andrew Tolonen

Article

Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

Insert

Plac-RFP (IPTG-inducible red fluorescent protein)

Expression

Bacterial

Promoter

Plac

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pW228-lenti-sg1-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189944

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg1-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW229-lenti-sg2-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189945

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg2-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW230-lenti-sg3-mmSerpinh1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189946

Purpose

Lentiviral vector to co-express a mouse Serpinh1 spsgRNA (sg3-Serpinh1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Serpinh1 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pW317-lenti-sg1-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189947

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg1-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW318-lenti-sg2-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189948

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg2-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pW319-lenti-sg3-mmItga9-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189949

Purpose

Lentiviral vector to co-express a mouse Itga9 spsgRNA (sg3-Itga9) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itga9 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pW401-lenti-sg1-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189950

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg1-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #1

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pW402-lenti-sg2-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189951

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg2-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #2

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pW403-lenti-sg3-mmItgb1-pEF1s-NLS-mScarlet-I-P2A-BlastR

Plasmid

#189952

Purpose

Lentiviral vector to co-express a mouse Itgb1 spsgRNA (sg3-Itgb1) with NLS-mScarlet-I

Depositor

Kenneth Yamada

Article

Sekiguchi et al J Dent Res. 2023 Feb;102(2):197-206. doi

Insert

NLS-mScarlet-I-P2A-BlastR; Itgb1 spsgRNA #3

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K216E]

Plasmid

#188600

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K216E [AAA=> GAA]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K276A]

Plasmid

#188597

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K276A [AAG=>GCG]

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[K284A]

Plasmid

#188595

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K284A [AAG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A]

Plasmid

#188594

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[K187A]

Plasmid

#188593

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

K187A [AAA=> GCA]

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr-CM

Plasmid

#188585

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler Catalytic mutant

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr

Plasmid

#188584

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAFMW-Nbr[N1-383]

Plasmid

#188586

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler N-terminal domain

Tags

FLAG-Myc

Expression

Insect

Mutation

deleted first ATG

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_316

Plasmid

#180547

Purpose

Entry vector containing sfGFP

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

sfGFP

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_344

Plasmid

#180548

Purpose

Entry vector containing cjBlue

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

cjBlue

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-ammB3

Plasmid

#167815

Purpose

Expresses His6-tagged AmmB3 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmB3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-ammC1

Plasmid

#167816

Purpose

Expresses His6-tagged AmmC1 in E. coli

Depositor

Wilfred van der Donk

Article

Daniels et al Nat Chem. 2022 Jan;14(1):71-77. doi: 10.

Insert

AmmC1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_345

Plasmid

#180550

Purpose

Entry vector containing eforRed

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

eforRed

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_036

Plasmid

#180558

Purpose

Entry vector containing L3S1P13 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P13 terminator

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_009

Plasmid

#180518

Purpose

Empty backbone for D1.1 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_137

Plasmid

#180521

Purpose

Empty backbone for D1.2 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_010

Plasmid

#180519

Purpose

Empty backbone for D1.2 cloning, contains LacZ dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_040

Plasmid

#180562

Purpose

Entry vector containing L3S2P24 terminator

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S2P24 terminator

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_048

Plasmid

#180564

Purpose

Entry vector containing L3S1P00

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

L3S1P00_E1.4b

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge