We narrowed to 28,470 results for: LEA

1. pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

   Plasmid

   #134661

   Purpose

   Expresses EGFP-tagged synaptic vesicle protein

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   vesicle-associated membrane protein 2 (VAMP2 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   March 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pS1643 (pFA6-NATMX-CDC42pr-yGFP1-10)

   Plasmid

   #86420

   Purpose

   N-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter

   Depositor

   Sue Jaspersen

   Article

   Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

   Insert

   CDC42pr-GFP1-10
   

   Tags

   GFP1-10

   Expression

   Yeast

   Available Since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRII_TOPO_hNEAT1

   Plasmid

   #61518

   Purpose

   human NEAT1_v1 short isoform 3.7kb insert in general cloning vector

   Depositor

   Archa Fox

   Article

   Clemson et al Mol Cell. 2009 Mar 27;33(6):717-26. doi:

   Insert

   NEAT1 (NEAT1 Human)
   

   Use

   General cloning vector

   Mutation

   *see comment below

   Available Since

   March 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRE-KRAB-dCas9-IRES-BFP

   Plasmid

   #85449

   Purpose

   Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-CMV-mCherry-T2A-GFP

   Plasmid

   #109427

   Purpose

   A lentiviral backbone expressing mCherry-T2A-eGFP off of a CMV promoter.

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry T2A GFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-PV-NLS-GFP

   Plasmid

   #17300

   Depositor

   Anton Bennett

   Article

   Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

   Insert

   PV-GFP-NLS (Pvalb Rat)
   

   Tags

   Myc and NLS

   Expression

   Mammalian

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

   Plasmid

   #228726

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D56R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

   Plasmid

   #228725

   Purpose

   Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

    Plasmid

    #228729

    Purpose

    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R

    Plasmid

    #228727

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R

    Plasmid

    #228728

    Purpose

    Expresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R

    Plasmid

    #228730

    Purpose

    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. TDP-43 mRuby

    Plasmid

    #216225

    Purpose

    TDP-43 with a C-terminal mRuby tag for expression in mammalian cells.

    Depositor

    Jennifer Lee

    Article

    Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-hSynapsin-Cre-WPRE

    Plasmid

    #86641

    Purpose

    Expresses Cre in mammalian cells

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    Cre
    

    Use

    Cre/Lox, Lentiviral, Mouse Targeting, and Synthet…

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Hy_pMT Flag-URH49 WT

    Plasmid

    #110127

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A)

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    April 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a_Pro-IL-1β

    Plasmid

    #214340

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL1B (IL1B Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid

    #228724

    Purpose

    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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