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pcDNA3.1-CHRFAM7ADelta2bp-mGFP

Plasmid

#62636

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mEGFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Tags

mEGFP inserted in M3-M4 loop

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A ca…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
Hy_pMT Flag-UAP56 MUT

Plasmid

#110124

Purpose

Expresses Flag-tagged human UAP56 (DDX39B) with 4 mutations that impact circRNA nuclear export activity

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Tags

Flag

Expression

Insect

Mutation

4 mutations (KSLN motif changed to RSFS)

Promoter

Metallothionein Promoter (pMT)

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pJC-ase0.8kb-TALE3-VP64-P10

Plasmid

#104610

Purpose

Expresses TALE3 under the control of a 0.8kb ase enhancer element

Depositor

Tudor Fulga

Article

Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

Insert

0.8kb ase enhancer element (ase Fly)

Use

TALEN

Expression

Insect

Promoter

hsp70

Available Since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CHRFAM7ADelta2bp-mCherry

Plasmid

#62638

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with mCherry fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-C12AC15A-LIN-41

Plasmid

#52725

Purpose

retroviral expression of human LIN-41 / TRIM71 containing cysteine to alanine point mutations at cysteine 12 and 15 and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed cysteines 12 and 15 to alanines

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMKO.1-puro/hFOXH1 shRNA3

Plasmid

#59308

Purpose

Knockdown of human FOXH1

Depositor

Shinya Yamanaka

Article

Takahashi et al Nat Commun. 2014 Apr 24;5:3678. doi: 10.

Insert

hFOXH1 shRNA (FOXH1 Human)

Use

RNAi and Retroviral

Promoter

hU6

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
RSV/mGATA3 (sense)

Plasmid

#83816

Purpose

Mammalian expression of mouse GATA3

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3 (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pMXs-hs-3xHA-deltaBBox-LIN-41

Plasmid

#52719

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the BBox domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 194-320

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-3xHA-deltaFilamin-LIN-41

Plasmid

#52721

Purpose

retroviral expression of human LIN-41 / TRIM71 containing deletion of the Filamin domain and 3 N-terminal HA tags

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

LIN-41 (TRIM71 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

deleted amino acids 483–583

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pDD3233 pCS2-sfCherry11Mx4-10aa-zH2B-SV40NLS-sfGFP-NPLnls-p2a-TEVp

Plasmid

#251771

Purpose

Nuclear reporter for fluorescent protein reconstitution assay-p2a-TEVp

Depositor

Dave Dingal

Article

Lim Suan et al Nat Commun 17, 3257 (2026).

Insert

H2B (hist2h2l Zebrafish)

Tags

4x sfCherry3C small fragments, NLS (SV40) and NLS…

Expression

Mammalian

Promoter

CMV IE94

Available Since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pTA-M+

Plasmid

#249818

Purpose

Expresses Mycobacterium tuberculosis topoisomerase I under the control of the Tet promoter in mycobacteria

Depositor

Yuk-Ching Tse-Dinh

Article

Sandhaus et al Antimicrob Agents Chemother. 2016 Jun 20

Insert

Mycobacterium tuberculosis topoisomerase I (topA Mycobacterium tuberculosis)

Tags

6xHis

Expression

Bacterial

Promoter

Tet

Available Since

March 9, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gCxl5-CBh-mCherry-SV40late

Plasmid

#249120

Purpose

expression of gCxcl5 and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gCxcl5 (Cxcl5 Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gPdpn-CBh-mCherry-SV40late

Plasmid

#249123

Purpose

expression of gPdpn and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gPdpn (Pdpn Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pscAAV-U6-gSCR-CBh-mCherry-SV40late

Plasmid

#249124

Purpose

expression of gSCR (scrambled, non-targeting gRNA) and mCherry

Depositor

Matthew Krummel

Article

Kuhn et al J Exp Med. 2026 May 4;223(5):e20251228.

Insert

gSCR (Scr Mouse)

Use

AAV

Promoter

hU6

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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SNAIL-Luc

Plasmid

#250936

Purpose

Lentiviral vector encoding a truncated promoter region of human SNAIL gene driving a luciferase reporter

Depositor

Michelle Farkas

Article

Chhe et al Clocks Sleep. 2025 Sep 28;7(4):54. doi:

Insert

SNAIL promoter (SNAI1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Contains a 340 bp region of the truncated SNAIL p…

Promoter

SNAIL

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-HA-UBA7-Trp311*

Plasmid

#251244

Purpose

Expression of UBA7 with nonsense mutation (Trp311 Stop)

Depositor

Francisco Bustos

Article

Bandi et al iScience 2026. doi: 10.1016/j.isci.2026.

Insert

UBA7 (UBA7 Human)

Tags

HA

Expression

Mammalian

Mutation

Trp311*

Promoter

CAG

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-NLS-delta_VHL54:213-miniTurbo-3xFLAG

Plasmid

#248672

Purpose

To generate inducible Flp-In cells expressing Cterm tagged miniTurbo delta VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL(54-213) (VHL Human)

Tags

FLAG-miniTurbo and NLS

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCDNA5-FRT/TO-3XFLAG-miniTurbo-NLS-VHL

Plasmid

#248669

Purpose

To generate inducible Flp-In cells expressing Nterm tagged miniTurbo full length VHL for ProtacID.

Depositor

Cheryl Arrowsmith

Article

Shrestha et al Nat Commun. 2025 Aug 29;16(1):8089. doi:

Insert

VHL (VHL Human)

Tags

FLAG-miniTurbo-NLS

Expression

Mammalian

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV-N-RAM-CytoTape-V5

Plasmid

#239425

Purpose

CytoTape signal monomer for recording Npas4 protein activity

Depositor

Changyang Linghu

Article

Zheng et al Nature. 2026 Jan 26. doi: 10.1038/s41586

Insert

CytoTape-V5

Use

AAV

Tags

V5-dMBP

Expression

Mammalian

Promoter

N-RAM promoter

Available Since

Nov. 12, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBBR1-EcTyrRS(VSMA,D165G)

Plasmid

#231132

Purpose

Expresses mutant E. coli TyrRS from a derepressed lac promoter for proteome-wide incorporation of tyrosine in E. coli/K. pneumoniae.

Depositor

Abhishek Chatterjee

Article

Loynd et al Nat Chem Biol. 2025 Oct 2. doi: 10.1038/

Insert

E. coli tyrosyl tRNA synthetase

Use

Klebsiella pneumoniae expression

Expression

Bacterial

Mutation

Y37V, D165G, D182S, P183M, L186A, D265R

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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