We narrowed to 18,351 results for: multi
-
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsapphireAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pblu-AAVS1-Cas9-p300-M2rtTA-AAVS1
Plasmid#112261PurposeDonor plasmid with a Cas9-p300 expression cassette, a M2rtTA expression cassette, a T2A-puro selection cassette, and two gRNA recognition sequences at both ends of the whole insertion DNA fragment.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertspCas9-p300 (EP300 Human, Synthetic)
ExpressionMammalianAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221A
Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC19-E6MBW6
Plasmid#103102PurposeCas9 coding gene template with optimized sequence for human codon usageDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertE6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
UseCRISPR; Cloning vectorMutationhuman codon-optimizedAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQZ210
Plasmid#106414PurposeCo-expresses trans SNARE complex with pDC183 in E.coliDepositorInsertsTagsHis tagExpressionBacterialMutationFragment 28-66, Codon optimization and Fragment 1…PromoterT7Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-Neo-GFP/V5-Foxa1-(P2A)-Flag-Gata5
Plasmid#105509PurposeMSCV-driven retroviral Foxa1 and Gata5 expression (P2A-linked, V5 and Flag-tagged each)DepositorAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE
Plasmid#101108PurposeEncodes dCas9(N) fused to BDKRB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV dCas9-MC (eGFP)
Plasmid#89931PurposeExpresses the dCas9-MC fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9-MC
TagsFlagExpressionMammalianMutationdeactivated Cas9, fragment of M.SssI (residues 27…PromoterCMVAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 Ub-CRT
Plasmid#69619PurposeExpress cytoplasmic mature CRT.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitina-Calreticulin (mature)
TagsEGFP and UbiquitinExpressionMammalianMutationDeletion of signal peptide of calreticulin at the…PromoterCMVAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR-Blunt II-TOPO-PTPRD
Plasmid#29705DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseCloning vectorMutationnoneAvailable sinceMay 26, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMBEC8
Plasmid#187599PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-MCS-WPRE-UbC-Emerald
Plasmid#211796PurposeLentiviral vector plasmid containing a multiple cloning site (MCS) under the spleen focus-forming virus (SFFV) promoter and expressing Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsmultiple cloning site
Emerald
UseLentiviralExpressionMammalianPromoterSFFV and UbCAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRU51
Plasmid#167677PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU52
Plasmid#167678PurposeFinal destination vector for single Gateway LR reactionDepositorInsertPcUB4-2::spCas9
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Omega 2
Plasmid#118047PurposeGoldenBraid2.0 Omega 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…ExpressionBacterial, Insect, and Mamm…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
Alpha2
Plasmid#118045PurposeGoldenBraid2.0 Alpha 2 destination vectorDepositorTypeEmpty backboneUseSynthetic Biology; Assembly of gb2.0 transcriptio…ExpressionBacterial, Insect, and Mamm…Available sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGE1108
Plasmid#202772PurposeCRISPR-KO in Arabidopsis thalianaDepositorInsertsFAST marker
pRPS5a:zCas9_ttriple
ccdB cassette (BsaI-excisable)
UseCRISPRExpressionPlantAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMiniP
Plasmid#118049PurposeGBPart - Minimal Promoter for expression in mammalian cellsDepositorInsertminimal TATA-box promoter with low basal activity
UseSynthetic BiologyExpressionMammalianAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
iMb-Control-Mosaic (IR98.10)
Plasmid#99748PurposeRosa26 gene targeting vector to induce a Cre-dependent mosaic of cells expressing different fluorescent and marker proteins localized to the membraneDepositorInsertMbYFP, MbTomato, MbKate2
UseCre/Lox and Mouse TargetingExpressionMammalianAvailable sinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_AR-RE_MLPmin_BC1011-luc2
Plasmid#227106PurposeAndrogen receptor response element for luciferase and barcode assays; barcode BC1011DepositorInsert8x clustered AR response element linked to MLP minimal promoter driving barcode 1011 and a luciferase reporter gene
UseAAVExpressionMammalianAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only