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AiP15285 - pAAV-AiE0170h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN5285)

Plasmid

#230509

Purpose

AiE0170h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP15263 - pAAV-AiE0219h-minBG-iCre(R297T)-BGHpA (Alias: CN5263)

Plasmid

#230406

Purpose

AiE0219h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate PRVABC59 NS3 helicase domain

Plasmid

#228664

Purpose

Bacterial expression of the Zika virus NS3 helicase

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus NS3 helicase

Tags

His-GST-TEV

Expression

Bacterial

Available since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pZa pBAD csgA CBDcex pLac Ara csgGEF

Plasmid

#226378

Purpose

Plasmid carries csgA and csgG,E,F genes of curli operon

Depositor

Urartu Seker

Publication

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

csgA and csgG,E,F genes of curli operon

Expression

Bacterial

Promoter

pBAD

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pJL NarX Myc

Plasmid

#225651

Purpose

Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

Depositor

Neha Kamat

Publication

Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

Insert

NarX (narX E. coli)

Use

Cell free expression

Tags

Myc

Expression

Bacterial

Promoter

T7

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12040 - pAAV-3xSP10ins-AiE0354m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2040)

Plasmid

#223971

Purpose

AiE0354m is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Publication

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CAG_mCh_p85a_mCh

Plasmid

#199630

Purpose

FRET acceptor for IGF1R sensor

Depositor

Ryohei Yasuda

Publication

Tu et al Sci Adv. 2023 Aug 2;9(31):eadg0666. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p85a-SH2 (Pik3r1 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

Plasmid

#218513

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

Depositor

Emmanuelle Charpentier

Publication

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of the Plac_mrfp cassette

Promoter

PgyrA_ermBL-ermB'

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-Spy-CD86

Plasmid

#223598

Purpose

Lentiviral expression of human CD86 with SpyTag003 in mammalian cells

Depositor

Enfu Hui

Publication

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

Spytag003

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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ePPEmax*

Plasmid

#205244

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Publication

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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PH-PLCd1-mNGx3

Plasmid

#202753

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PLCD1v2(1-170): mNG2:mNG2:mNG2 (PLCD1 Human)

Expression

Mammalian

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CRY2-mCherry

Plasmid

#178525

Purpose

Expresses CRY2-mCherry in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRY2-mCherry

Expression

Mammalian

Promoter

CAG

Available since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_hsHUR-Halo

Plasmid

#175303

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (ELAVL1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_mmHUR-Halo

Plasmid

#175311

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HUR (Elavl1 Mouse)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Publication

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC2-GPT2-3xFLAG

Plasmid

#163447

Purpose

lentiviral overexpression vector for human GPT2

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GPT2 (GPT2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

CMV

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTagBFP2-C1-lynN11-FKBP-PI-PLCN187

Plasmid

#139308

Purpose

N-terminal half of split PI-PLC targeted to plasma membrane

Depositor

Gerry Hammond

Publication

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LYN(1-11):RSANS[GA]4:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Expression

Mammalian

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgGrin2b

Plasmid

#124864

Purpose

Mutagenesis of Grin2b

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin2b (Grin2b Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYC deltab_P2A_Hygro_Barcode

Plasmid

#120507

Purpose

Barcoded lentiviral vector to express MYC deltab in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYC deltab (MYC Human)

Use

Lentiviral

Mutation

Deletion of basic motif

Promoter

EF1a

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Publication

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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