We narrowed to 5,551 results for: crispr cas9 grna plasmid
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Plasmid#138135PurposeTargeted simultaneous C-to-T and A-to-G in riceDepositorInsertAPOBEC3A-wtTadA-TadA7.10-nCas9-UGI-NLS
ExpressionPlantAvailable SinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-gRNA
Plasmid#158973PurposeA human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.DepositorInsertgRNA3
ExpressionMammalianPromoterU6Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
gRNA-hIRF-1 #12/pSIR-hCD2
Plasmid#135392PurposeExpresses a guide RNA (gRNA) to target human IRF-1 promoterDepositorInsertgRNA_hIRF1 promoter #12
UseCRISPR and RetroviralExpressionMammalianPromoterU6Available SinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
SunTag-FWAgRNA-4-14aa-TET1cd
Plasmid#106436PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS (TET1 Synthetic, Human, Mustard Weed)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9
Plasmid#223163Purpose2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationEncodes aa 204-310 (MECP2)Available SinceAug. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQdCas9.luxR(mut)-sggfp
Plasmid#236185PurposeThe plasmid pQdCas9.luxR(mut)-sggfp expresses the dCas9 endonuclease and the sgRNA targeting the GFP gene. Additionally, this plasmid contains a mutation in the luxR gene.DepositorInsertQuorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas9, and sgRNA for GFP gene
PromoterQuorum sensing promoterAvailable SinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry
Plasmid#170182PurposeMammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptideDepositorInsertmCherry
Tags3XFlag and T2AExpressionMammalianPromoterCBhAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV.U6.gDNMT1.Cas9-T2A-GFP
Plasmid#170362PurposePlasmid used for Crispr/cas9 based disruption of human DNMT1.DepositorInsertCas9-T2A-GFP
UseLentiviralAvailable SinceJune 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
B-HiFi SpCas9
Plasmid#207362PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HiFi SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterCBhAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-Sniper SpCas9
Plasmid#207361PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-Sniper SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationF539S, M763I, K890N, amino acids 1005-1013 replac…PromoterCBhAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only