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pDual_dsCas9_Blast_hs_NC_chr1

Plasmid

#214690

Purpose

Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

Depositor

Kevin Janes

Insert

dgRNA_Chr1

Use

CRISPR and Lentiviral

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_GFP

Plasmid

#221469

Purpose

Gateway cloning of GFP overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Gateway Cloning

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_nLightR

Plasmid

#217657

Purpose

Expresses norepinephrine indicator nLightR under mammalian promoter

Depositor

Tommaso Patriarchi

Publication

Kagiampaki et al Nat Methods. 2023 Sep;20(9):1426-1436. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

nLightR

Expression

Mammalian

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pKESa

Plasmid

#211828

Purpose

Low copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

Depositor

Anita Emmerstorfer-Augustin

Publication

Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

Insert

eGFP

Expression

Bacterial

Promoter

T5

Available since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Publication

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Expression

Insect

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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SP_H6_HaloK73T_KDEL_pCMV1

Plasmid

#183686

Purpose

Mammalian expression of ER-targeted metastable Halotag/folding sensor (HaloK73T)

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET30_Halotag_K73T_v2

Plasmid

#183692

Purpose

Bacterial expresssion of ER-targeted metastable Halotag/folding sensor HaloTag (K73T)

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo(K73T)

Expression

Bacterial

Mutation

K73T in Halotag

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDS2149

Plasmid

#184275

Purpose

Tet-on genome insertion system

Depositor

Dominique Sanglard

Insert

CatrTA

Expression

Yeast

Promoter

TDH3

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XI3

Plasmid

#176157

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII1

Plasmid

#176159

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-XII5

Plasmid

#176161

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pJDC124

Plasmid

#157877

Purpose

mCherry is expressed under the mycobacterial phsp60 heat shock promoter for in vivo and in vitro detection.

Depositor

Jeffrey Cirillo

Publication

Kong et al PLoS One. 2016 Mar 2;11(3):e0149972. doi

Insert

mCherry

Expression

Bacterial

Promoter

phsp60

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCri-15b

Plasmid

#61336

Purpose

Protein expression.

Depositor

F Xavier Gomis-Ruth

Publication

Goulas et al PLoS One. 2014 Nov 11;9(11):e112643. doi

Insert

Green fluorescent protein

Tags

His-tag

Expression

Yeast

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTHSSd_29

Plasmid

#59959

Purpose

PTac alpha fragment expression

Depositor

Christopher Voigt

Publication

Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

Insert

alpha fragment

Expression

Bacterial

Mutation

N/A

Promoter

PTac

Available since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-MAVS(KaganF57)

Plasmid

#52000

Purpose

Expresses the human MAVS CDS containing "weak translational context"

Depositor

Jonathan Kagan

Publication

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEB602

Plasmid

#22071

Depositor

Emmanuelle Bouveret

Publication

Gully et al Proteomics. 2006 Jan . 6(1):282-93.

Type

Empty backbone

Tags

CBP

Expression

Bacterial

Available since

Oct. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pJBL7085

Plasmid

#167249

Purpose

orthogonal mRFP reporter output for cell-free expression

Depositor

Julius Lucks

Publication

Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

Insert

pAKSIRV-mRFP1

Use

Synthetic Biology

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_HaloK73T_cyto

Plasmid

#183687

Purpose

Mammalian expression of cytosolic metastable Halotag/folding sensor (Cyto HaloK73T)

Depositor

Edward Avezov

Publication

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

Cyto Halo(K73T)

Expression

Mammalian

Mutation

K73T in Halotag

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pEY13

Plasmid

#191019

Purpose

Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Publication

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

arrd-4 (ZK938.2 Nematode)

Expression

Worm

Mutation

None

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMCSGC-GB1

Plasmid

#186786

Purpose

E. coli expression vector containing N-terminal 6His-GB1-TEV fusion.

Depositor

Alexei Savchenko

Publication

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Expression

Bacterial

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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