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We narrowed to 20,408 results for: ENA;

Showing: 61 - 80 of 20408 results

  1. SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

    Plasmid
    #244219
    Purpose
    Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker
    Depositor
    Tim Reddy
    Insert
    dEnAsCas12a-VPR
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    dEnAsCas12a: E174R, S542R, K548R, and D908A
    Promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244219-plasmid-map-sequence-id-486074

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  2. SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

    Plasmid
    #244213
    Purpose
    Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker
    Depositor
    Tim Reddy
    Insert
    dEnAsCas12a-KRAB
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    dEnAsCas12a: E174R, S542R, K548R, and D908A
    Promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244213-plasmid-map-sequence-id-486067

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  3. SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

    Plasmid
    #244216
    Purpose
    Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker
    Depositor
    Tim Reddy
    Insert
    dEnAsCas12a-P300
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    dEnAsCas12a: E174R, S542R, K548R, and D908A
    Promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244216-plasmid-map-sequence-id-486065

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  4. SM280_EF1A-dEnAsCas12a-4xSID-T2A-BlastR

    Plasmid
    #244215
    Purpose
    Mammalian expression of dEnAsCas12a-4xSID with Blasticidin resistance selectable marker
    Depositor
    Tim Reddy
    Insert
    dEnAsCas12a-4xSID
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    dEnAsCas12a: E174R, S542R, K548R, and D908A
    Promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244215-plasmid-map-sequence-id-486060

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  5. SM279_EF1A-dEnAsCas12a-4xSID-T2A-EGFP

    Plasmid
    #244214
    Purpose
    Mammalian expression of dEnAsCas12a-4xSID with EGFP selectable marker
    Depositor
    Tim Reddy
    Insert
    dEnAsCas12a-4xSID
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    dEnAsCas12a: E174R, S542R, K548R, and D908A
    Promoter
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    244214-plasmid-map-sequence-id-486059

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  10. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

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  16. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Use
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Promoter
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

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  18. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T7Sequenase (JO584)

    Plasmid
    #208955
    Purpose
    A variant CE1 construct with T7 Sequenase DNA polymerase, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-Sequenase-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A)
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208955-plasmid-map-sequence-id-415744

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  20. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

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Showing: 61 - 80 of 20408 results