We narrowed to 22,468 results for: TES

1. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

   Plasmid

   #223065

   Purpose

   Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
   

   Use

   CRISPR

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Mutation

   Three regions of SpCas9 encode type IIS restricti…

   Promoter

   Dual T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV CMV-driven AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120297

   Purpose

   AAV vector for expression of AcrIIA4 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-LacO-Halo-RGD-PILATeS (200nM, Force Inert)

   Plasmid

   #216717

   Purpose

   Expresses HaloTag-RGD-PILATeS Force Inert Control with 200 nM BiT tag in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   HaloTag-RGD-PILATeS
   

   Tags

   6xHis and HaloTag

   Expression

   Bacterial

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. T7-LacO-Halo-RGD-PILATeS (700pM, Force Inert)

   Plasmid

   #216715

   Purpose

   Expresses HaloTag-RGD-PILATeS Force Inert Control with 700 pM BiT tag in bacteria

   Depositor

   Alex Dunn

   Article

   Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

   Insert

   HaloTag-RGD-PILATeS
   

   Tags

   6xHis and HaloTag

   Expression

   Bacterial

   Available Since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV CMV-driven AcrIIA4-2xmiR-1 target sites

   Plasmid

   #120299

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-1 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-1 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV CMV-driven AcrIIC1-scaffold (2xBsmBI sites)

   Plasmid

   #120300

   Purpose

   AAV vector for expression of AcrIIC1 (no miR binding sites, control vector)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIC1
   

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

   Plasmid

   #62579

   Purpose

   Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

   Depositor

   Michael Ruppert

   Article

   Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Luciferase

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)

   Plasmid

   #48307

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

   Plasmid

   #48283

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120298

    Purpose

    AAV vector for expression of AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIA4-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

11. -962 human cyclin D1 promoter TCF (0)-(4) sites mutant pGL3Basic

    Plasmid

    #32737

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Mutation

    -539 TCF(0) ATGAAAG deletion; -75 TCF(1) and -68 …

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

12. -962 human cyclin D1 promoter TCF (1)-(4) sites mutant pGL3Basic

    Plasmid

    #32736

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

13. -962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

    Plasmid

    #32734

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 promoter (CCND1 Human)
    

    Use

    Luciferase

    Mutation

    -75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

    Plasmid

    #120294

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. -962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic

    Plasmid

    #32735

    Depositor

    Frank McCormick

    Article

    Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

    Insert

    CCND1 Promoter (CCND1 Human)
    

    Use

    Luciferase

    Mutation

    -75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…

    Promoter

    -962 CCND1

    Available Since

    Jan. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

16. AAV CMV-driven AcrIIC1-2xmiR-122 target sites

    Plasmid

    #120302

    Purpose

    AAV vector for expression of AcrIIC1 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC1-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV CMV-driven AcrIIC3-2xmiR-122 target sites

    Plasmid

    #120303

    Purpose

    AAV vector for expression of AcrIIC3 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    AcrIIC3-2xmiR-122 binding sites
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

18. pFastBac LIC cloning vector with BioBrick polycistronic restriction sites (11A)

    Plasmid

    #48294

    Depositor

    Scott Gradia

    Article

    Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Nov. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET His6 TEV expression vector with BioBrick polypromoter restriction sites (14-B)

    Plasmid

    #48308

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK94 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (KanR)

    Plasmid

    #179078

    Purpose

    Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    S. pyogenes Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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