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Showing: 61 - 80 of 7280 results

  1. B. subtilis Single Gene Deletion Library--Kanamycin

    Plasmid Kit
    #1000000115
    Purpose
    B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
    Depositor
    Carol Gross
    Available since
    July 24, 2017
    Availability
    Academic Institutions and Nonprofits only

  2. B. subtilis CRISPRi Essential Gene Knockdown Library

    Plasmid Kit
    #1000000116
    Purpose
    B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
    Depositor
    Carol Gross
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  3. Click editor utilizing Cdc13(Y556A) for template recruitment - pCMV-T7-Cdc13(Y556A)-nCas9-EcKlenow (JO1329)

    Plasmid
    #217797
    Purpose
    Variant CE1 construct with Cdc13(Y556A) telomere binding protein for template recruitment, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Cdc13(Y556A)-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Cdc13(Y556A); nSpCas9(H840A); EcKlenow(-exo;D355A…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217797-plasmid-map-sequence-id-432864

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  4. Click editor utilizing mSA for template recruitment and ePhi29 DNA polymerase - pCMV-T7-mSA-nCas9-ePhi29 (JO1398)

    Plasmid
    #217803
    Purpose
    Variant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    mSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…
    Promoter
    CMV and T7
    Available since
    Sept. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217803-plasmid-map-sequence-id-432890

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  6. KSHV Tiling Library

    Pooled Library
    #180272
    Purpose
    sgRNA library tiling entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes viral genome. A guide every ~8 bp allows pooled screening of viral genome.
    Depositor
    Britt Glaunsinger
    Article
    Morgens et al PLoS Pathog. 2022 Jan 18;18(1):e1010236.
    Expression
    Mammalian
    Species
    Kaposi’s sarcoma-associated herpes virus
    Use
    Lentiviral
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only

  14. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 2 (positions 437-527)

    Pooled Library
    #174296
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library N501Y Tile 1 (positions 333-436)

    Pooled Library
    #174295
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD N501Y variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  16. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 2 (positions 437-527)

    Pooled Library
    #174294
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 2, positions 437-527) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  17. SARS-CoV-2 Spike (S) Receptor Binding Domain (RBD) library E484K Tile 1 (positions 333-436)

    Pooled Library
    #174293
    Purpose
    This library contains mutations to SARS-CoV-2 Spike RBD E484K variant in which all surface exposed residues on S RBD (Tile 1, positions 333-436) were mutated to every other amino acid.
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Expression
    Yeast
    Available since
    Sept. 13, 2021
    Availability
    Academic Institutions and Nonprofits only

  18. pcDNA3-TLR1-YFP

    Plasmid
    #13014
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 1 (TLR1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13014-plasmid-map-sequence-id-237573

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  19. pcDNA3-TLR1-YFP

    Plasmid
    #13014
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 1 (TLR1 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13014-plasmid-map-sequence-id-237573

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  20. hTLR1 flag

    Plasmid
    #13081
    Depositor
    Ruslan Medzhitov
    Insert
    TLR1 (TLR1 Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Amino acids 1-18 have been replaced with mammalia…
    Promoter
    Available since
    Oct. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
Showing: 61 - 80 of 7280 results