Addgene

We narrowed to 6,039 results for: itch

Showing: 61 - 80 of 6039 results

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pX330-PITCh-PXN

Plasmid

#227315

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PXN (PXN Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CLTC

Plasmid

#227312

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CLTC (CLTC Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgVRK1

Plasmid

#199638

Purpose

Tamoxifen-inducible expression of sgRNA targeting VRK1

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A (VRK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-GOLGA2

Plasmid

#207791

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pUC19-Mu-switch-R-loop

Plasmid

#134899

Purpose

Generation and purification of R-loops for in vitro biochemistry

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Insert

mouse IgH switch repeat region

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Jan. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-TUBB4B

Plasmid

#207785

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of TUBB4B (TUBB4B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H3C2

Plasmid

#207780

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H3C2 (H3C2 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgCh2-2

Plasmid

#199637

Purpose

Tamoxifen-inducible expression of sgRNA control targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-PEX3

Plasmid

#227303

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of PEX3 (PEX3 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-MAPRE1

Plasmid

#207793

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-MAP4

Plasmid

#227295

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of MAP4 (MAP4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-CEP192

Plasmid

#227288

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of CEP192 (CEP192 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-ARL13B

Plasmid

#227276

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of ARL13B (ARL13B Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p223-Pulse-Switch

Plasmid

#217889

Purpose

Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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p213-SWITCH-OFF

Plasmid

#217888

Purpose

Retroviral Switch-OFF vector for sgRNA expression; U6-BbsIx2-SWITCH-OFF-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103 ITCH WW domain #3

Plasmid

#104218

Purpose

Bacterial expression of WW domain #3 from ITCH

Depositor

Marius Sudol, Sachdev Sidhu

Insert

ITCH WW domain #3 (ITCH Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 ITCH WW domain #4

Plasmid

#104219

Purpose

Bacterial expression of WW domain #4 from ITCH

Depositor

Marius Sudol, Sachdev Sidhu

Insert

ITCH WW domain #4 (ITCH Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 ITCH WW domain #1

Plasmid

#104216

Purpose

Bacterial expression of WW domain #1 from ITCH

Depositor

Marius Sudol, Sachdev Sidhu

Insert

ITCH WW domain #1 (ITCH Human)

Use

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 61 - 80 of 6039 results

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