Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 1,717 results for: plasmid for e coli

Showing: 61 - 80 of 1717 results

  1. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

    Enlarge

  2. p416-GPD-PPK(mutant)

    Plasmid
    #183942
    Purpose
    Expresses catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli )
    Use
    Tags
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183942-plasmid-map-sequence-id-363372

    Enlarge

  3. PCDF Bravo-PotH-OL1del

    Plasmid
    #216749
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 1 (OL1) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    potH (potH E. coli (Bacteria))
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 1 (OL1) with the sequence of [FKISLAEM…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216749-plasmid-map-sequence-id-434430

    Enlarge

  4. PCDF Bravo-PotH-OL2del

    Plasmid
    #216750
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 2 (OL2) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    potH (potH E. coli (Bacteria))
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216750-plasmid-map-sequence-id-434427

    Enlarge

  5. PCDF Bravo-PotH-OL3del

    Plasmid
    #216751
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the role of the outer loop 3 (OL3) by examining the variant lacking OL1 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    potH (potH E. coli (Bacteria))
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 3 (OL3) with the sequence of [ELLGGPDS…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216751-plasmid-map-sequence-id-434425

    Enlarge

  6. PCDF Bravo-PotH-OL3/2sub

    Plasmid
    #216752
    Purpose
    Study the interaction of PotH in E. coli, specifically focusing on the variant in which outer loop 2 (OL2) is substituted with OL3 in its interaction with exogenous peptides.
    Depositor
    Gordon Laurie
    Insert
    potH (potH E. coli (Bacteria))
    Use
    Tags
    10x His
    Expression
    Bacterial
    Mutation
    Outer loop 2 (OL2) with the sequence of [WMGILKNN…
    Promoter
    Available since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    216752-plasmid-map-sequence-id-434424

    Enlarge

  16. pJBEI-6409

    Plasmid
    #47048
    Purpose
    BglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47048-plasmid-map-sequence-id-67070

    Enlarge

  17. pJBEI-6410

    Plasmid
    #47049
    Purpose
    BglBrick plasmid (=pBbA5a-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coli
    Depositor
    Taek Soon Lee
    Article
    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1
    Insert
    Codon-optimized sequences for MEV pathway expression in E. coli to produce limonene
    Use
    Synthetic Biology; Bglbrick
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PlacUV5
    Available since
    Sept. 12, 2013
    Availability
    Academic Institutions and Nonprofits only
    47049-plasmid-map-sequence-id-408250

    Enlarge

  18. p416-GPD-HA-PPK(mutant)

    Plasmid
    #183944
    Purpose
    Expresses HA-tagged catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183944-plasmid-map-sequence-id-363303

    Enlarge

  19. pTF-ahpC-rfp

    Plasmid
    #153035
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153035-plasmid-map-sequence-id-318838

    Enlarge

  20. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

    Enlarge

Showing: 61 - 80 of 1717 results