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pLVX-CMV-Galpha-i3 G183S ONE-GO

Plasmid

#204338

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3 G183S ONE-GO

Use

Lentiviral

Mutation

G183S mutation in Galpha-i3 (renders it RGS insen…

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-myc-GBAi

Plasmid

#171753

Purpose

For the mammalian expression of myc-GBAi

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

Nov. 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR_Hu_Plk5(WT)

Plasmid

#136329

Purpose

Human Plk5 WT in Gateway pENTR

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Use

Gateway entry vector

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLIC-His-GBAi

Plasmid

#171755

Purpose

For the expression of His-GBAi in bacteria

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

His

Expression

Bacterial

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p3xFLAG-CMV-14-GINIP-Nluc W139A

Plasmid

#223557

Purpose

Plasmid expressing GINIP-Nluc W139A in mammalian cells

Depositor

Mikel Garcia-Marcos

Article

Luebbers et al Sci Signal. 2025 Mar 25;18(879):eadp6457

Insert

human GINIP W139A - linker(GGGS) - Nluc

Tags

3xFLAG

Expression

Mammalian

Mutation

W139A mutation in GINIP sequence

Available Since

March 28, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204341

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-q ONE-GO

Plasmid

#189738

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-q ONE-GO
GNAQ (Gnaq Mouse)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i1 ONE-GO

Plasmid

#189735

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i1 ONE-GO
GNAI1 (Gnai1 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-i3 ONE-GO

Plasmid

#189737

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i3 ONE-GO
GNAI3 (Gnai3 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RSV-Flag-Brd4

Plasmid

#86616

Purpose

Flag tagged mouse Brd4 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd4 (Brd4 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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RSV-Flag-Brd2

Plasmid

#86614

Purpose

Flag tagged mouse Brd2 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd2 (Brd2 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i2 ONE-GO

Plasmid

#189736

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i2 ONE-GO
GNAI2 (Gnai2 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RSV-Flag-Brd3

Plasmid

#86615

Purpose

Flag tagged mouse Brd3 expression construct for cell transfection

Depositor

Mario García-Domínguez

Article

Garcia-Gutierrez et al J Cell Sci. 2012 Aug 1;125(Pt 15):3671-8

Insert

Brd3 (Brd3 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

RSV

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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CaTCH_empty-backbone

Plasmid

#157746

Purpose

Lentiviral CaTCH barcoding plasmid harboring an emtpy cloning site to insert a CaTCH barcoding library or individual barcode sequences

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral; Catch barcode cloning

Expression

Mammalian

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLIC-myc-GBAi W211A

Plasmid

#171754

Purpose

For the mammalian expression of myc-GBAi W211A

Depositor

Mikel Garcia-Marcos

Article

Leyme et al Proc Natl Acad Sci U S A. 2017 Nov 28;11

Insert

GBAi

Tags

myc

Expression

Mammalian

Available Since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLIC-His-RGS4

Plasmid

#204847

Purpose

Express His tagged-RGS4 in bacterial cells for protein purification

Depositor

Mikel Garcia-Marcos

Article

Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

Insert

RGS4 (RGS4 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204343

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTA231-p.Syn-PAmCherry1Hfx

Plasmid

#164661

Purpose

expression of H. volcanii codon-optimized PAmCherry1

Depositor

Anita Marchfelder

Article

Turkowyd et al Front Microbiol. 2020 Nov 20;11:583010.

Insert

PAmCherry1Hfx

Use

Archaeal expression

Mutation

codon-optimization for Haloferax volcanii

Promoter

p.Syn

Available Since

Feb. 20, 2021

Availability

Academic Institutions and Nonprofits only

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