We narrowed to 2,568 results for: CCS

1. pTBL3346 PB-nicking guide-blast

   Plasmid

   #226665

   Purpose

   PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

   Depositor

   Chang Liu

   Article

   Loveless et al bioRxiv 2021.11.05.467507

   Insert

   protective nicking sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Oct. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTBL435

   Plasmid

   #226628

   Purpose

   pcDNA-dTdT

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Insert

   catalytically deactivated TdT (DNTT Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   D343E, D345E

   Promoter

   CMV

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV_CMV_Prss-Halo-KDEL

   Plasmid

   #216256

   Purpose

   CMV promoter-regulated ER targeted HaloTag expression vector

   Depositor

   Edward Avezov

   Article

   Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

   Insert

   ER retained HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNeonGreen-C1-Nir1-631-894

   Plasmid

   #220089

   Purpose

   Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   NeonGreen-GGSGG

   Expression

   Mammalian

   Mutation

   amino acids 631-894

   Promoter

   CMV

   Available since

   June 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNeonGreen-N1-Nir1-613-630

   Plasmid

   #220088

   Purpose

   N-terminal amphipathic helix of Nir1-LNS2 domain; loses ability to bind PA

   Depositor

   Gerry Hammond

   Article

   Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

   Insert

   PITPNM3 (PITPNM3 Human)
   

   Use

   Tags

   GGSGG-NeonGreen

   Expression

   Mammalian

   Mutation

   amino acids 613-630

   Promoter

   CMV

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1+N-eGFP_SAMD9L_Asn885fs

   Plasmid

   #206175

   Purpose

   mammalian expression of SAMD9L Asn885fs

   Depositor

   Christopher Goodnow

   Article

   Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

   Insert

   SAMD9L (SAMD9L Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   N885Tfs*6

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1+N-eGFP_SAMD9L_Lys878fs

   Plasmid

   #206174

   Purpose

   mammalian expression of SAMD9L Lys878fs

   Depositor

   Christopher Goodnow

   Article

   Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

   Insert

   SAMD9L (SAMD9L Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K878Sfs*13

   Promoter

   CMV

   Available since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EK0544 CMV-TO-mCherry-SG(s70587-dblstop)SG-EGFP (FLP-IN)

   Plasmid

   #191176

   Purpose

   Mammalian expression of EK0209 with a second stop codon.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   CMV-TO:mCherry:s70587:EGFP (double-stop)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   additional stop codon

   Promoter

   CMV-TO

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. EK0546 SFFV-mCherry-SGc(GAPDH.s1-dblstop)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191178

   Purpose

   Mammalian expression of EK0493 with second stop codon.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:GAPDH.s1:EGFP (double-stop)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   additional stop codon

   Promoter

   SFFV

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EK0494 SFFV-mCherry-SGc(CFL1.s1)cSG-EGFP-(3xMS2) (FLP-IN)

    Plasmid

    #191181

    Purpose

    Expression of CFL1 sensor in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:CFL1.s1:EGFP-3xMS2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EK0290 SFFV-mCherry-SG(s70587-AND-s17d9b)SG-EGFP (FLP-IN)

    Plasmid

    #191143

    Purpose

    Expression of Sensor for (1 AND 2) "EK0208 AND EK0211" in mammalian cells; mCherry marker, EGFP output.

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:s70587-AND-s17d9b:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. EK0312 pAAV-EF1a-DIO-EYFP-WPRE-HGHpA

    Plasmid

    #191148

    Purpose

    AAV expression of a Cre reporter with EYFP output; derived from a gift from prof. Liqun Luo

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    DIO-EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    hEF1a

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. EK0341 SFFV-mCherry-SG(Bdnf.s1L153)SG-EGFP (FLP-IN)

    Plasmid

    #191150

    Purpose

    Expression of a 153 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L153:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. EK0358 SFFV-mCherry-SG(Bdnf.s1L36)SG-EGFP (FLP-IN)

    Plasmid

    #191152

    Purpose

    Expression of a 36 bp long sensor for murine Bdnf 3' UTR in mammalian cells

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:Bdnf.s1L36:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag

    Plasmid

    #186708

    Purpose

    N-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    His-Tag (6His)

    Expression

    Mutation

    Promoter

    PpsbA2*

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag

    Plasmid

    #186710

    Purpose

    N-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.

    Depositor

    Paula Tamagnini

    Article

    Mascia et al Green Chemistry, 24 (2022):6156

    Insert

    alr4766
    

    Use

    Synthetic Biology

    Tags

    His-Tag (6His)

    Expression

    Mutation

    Promoter

    Ptrc.x.tetO2

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lentiguide-Blast-eNMU710

    Plasmid

    #172658

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-eNMUA/pegRNA-eNMUB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PB-HD252

    Plasmid

    #172656

    Purpose

    Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

    Depositor

    Jay Shendure

    Article

    Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    pegRNA-HD252A/pegRNA-HD252B
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-sfGFP-DogTag Loop C

    Plasmid

    #171781

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Asp173 and Gly174

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-DogTag Loop C
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag and linkers inserted between residues Asp1…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28-sfGFP-SpyTag003 Loop B

    Plasmid

    #171777

    Purpose

    Expresses in bacterial cytoplasm superfolder GFP with SpyTag003 and flanking linkers between Asp102 and Asp103

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    sfGFP-SpyTag003 Loop B
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    SpyTag003 and linkers inserted between residues A…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge