We narrowed to 992 results for: DsRed-Express

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pSEED-mGreenLantern

Plasmid

#219643

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

Depositor

Markus Affolter

Publication

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern SEED Cassette

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-Dendra2

Plasmid

#219645

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

Depositor

Markus Affolter

Publication

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Dendra2 SEED Cassette

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pORF1-2

Plasmid

#206024

Purpose

An expression vector for mouse LINE1 ORF1-2

Depositor

Masamichi Kamihira

Publication

Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ORF1-2 derived from mouse LINE1

Expression

Mammalian

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int-BlastR

Plasmid

#199197

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors, with blasticidin resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int-BlastR

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int-G418R

Plasmid

#199198

Purpose

Insert to create split-intein Gal4[21-881]-C-int knock-in vectors, with G418 resistance, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int-G418R

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pTol2-HybridBarcode

Plasmid

#174037

Purpose

For Tol2 transgenesis of expressed barcode containing SpyCas9, SauCas9, and LbCas12a target sites

Depositor

James Gagnon

Publication

Takasugi et al Genetics. 2021 Nov 4. pii: 6420709. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CRISPR target sites

Use

Tol2 transgenesis

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV CaMKIIa ChR2 E90R D156N T159C 2A tDimer

Plasmid

#52878

Purpose

Chloride-conducting Channelrhodopsin with slow kinetics, neuron-specific promoter, plus red fluorescent protein, excitatory neurons

Depositor

Thomas Oertner

Publication

Wietek et al Science. 2014 Mar 27.

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Expression

Mammalian

Mutation

E90R, D156N, T159C

Promoter

CaMKIIa

Available since

July 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGC276

Plasmid

#19637

Depositor

Jane Hubbard

Publication

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available since

March 5, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGC163

Plasmid

#19635

Depositor

Jane Hubbard

Publication

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Gateway(R1-R2)

Use

Flp/frt, destination vector

Expression

Worm

Available since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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pR2HYG

Plasmid

#30170

Depositor

Lalita Ramakrishnan

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mycobacterium Strong Promoter (MSP)

Use

Mycobacteria expression

Tags

dsRed2

Available since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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nos-Cas9.874Z1

Plasmid

#112685

Purpose

Express hSpCas9 under nanos promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

nanos

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Ubiq-Cas9.874W

Plasmid

#112686

Purpose

Express hSpCas9 under Ubiquitin-63E promoter

Depositor

Omar Akbari

Publication

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

Ubiquitin-63E promoter

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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CSII-IDR2-humanTRIM5-FOS

Plasmid

#79066

Purpose

Express human TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM5alpha(human)-FOS (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Mammalian

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroX SgK269 WT

Plasmid

#68028

Purpose

expression of Sgk269 in mammalian cells

Depositor

Roger Daly

Publication

Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

Insert

PEAK1 (PEAK1 Human)

Use

Retroviral

Tags

FLAG/myc, HA, and IRES-DsRed

Expression

Mammalian

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pEN_TTRmiRc2

Plasmid

#25754

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE-tight promoter with dsRed2 coexpression.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

dsRed2

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xP3-GFP

Plasmid

#130277

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-GFP visible eye marker and multiple cloning site.

Depositor

Norbert Perrimon

Publication

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

3xP3-GFP

Use

CRISPR

Expression

Insect

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-CamKII-TRPV1-P2A-mCherry

Plasmid

#200829

Purpose

Transduce excitatory neurons with AAV to express TRPV1 along with an mCherry fluorescence reporter

Depositor

Hong Chen

Publication

Yang et al Brain Stimul. 2021 Jul-Aug;14(4):790-800

Insert

TRPV1-P2A-mCherry (Trpv1 Rat)

Use

AAV

Expression

Mammalian

Promoter

CamKII

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBAC-ECFP-15xQUAS_TATA-SV40

Plasmid

#104875

Purpose

piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

Depositor

Christopher Potter

Publication

Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

Type

Empty backbone

Expression

Insect

Available since

March 12, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDropIn-T2A-ZpGal4DBD

Plasmid

#199199

Purpose

Insert to create ZpGal4DBD knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-ZpGal4DBD

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-p65Zp

Plasmid

#199200

Purpose

Insert to create p65Zp knock-ins, via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Publication

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-p65Zp

Expression

Insect

Available since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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