We narrowed to 792 results for: NUA

1. pET28_6xHis-PARP1-E988K

   Plasmid

   #173945

   Purpose

   Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-E988K (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   V762A, E988K

   Promoter

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV1-SMAC-HA-eGFP

   Plasmid

   #67487

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pCMV4-SMAC-HA-eGFP

   Plasmid

   #67490

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCMV6-SMAC-HA-eGFP

   Plasmid

   #67492

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pCMV3-SMAC-HA-eGFP

   Plasmid

   #67489

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCMV2-SMAC-HA-eGFP

   Plasmid

   #67488

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCMV5-SMAC-HA-eGFP

   Plasmid

   #67491

   Purpose

   Targets HA-eGFP to mitochondrial intermembrane space (IMS)

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   SMAC (DIABLO Human)
   

   Use

   Tags

   HA-eGFP

   Expression

   Mammalian

   Mutation

   aa1-59

   Promoter

   attenuated CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. tet-pLKO-sgRNA-puro

   Plasmid

   #104321

   Purpose

   3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.

   Depositor

   Nathanael Gray

   Article

   Huang et al eLife. 2017 Sep 19;6. pii: e26693. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1/TO

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Control click editor - pCMV-T7-deadPCV2-nCas9-EcKlenow (EMK492)

   Plasmid

   #208943

   Purpose

   A control CE1 construct with catalytically attenuated PCV2 HUH, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   dPCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   PCV2(Y96F); nSpCas9(H840A); EcKlenow(-exo;D355A/E…

   Promoter

   CMV and T7

   Available Since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

    Plasmid

    #208945

    Purpose

    A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

    Promoter

    CMV and T7

    Available Since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Myristoylated Kinase Library

    Plasmid Kit

    #1000000012

    Purpose

    190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.

    Depositor

    William Hahn

    Available Since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

12. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit

    #1000000014

    Purpose

    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.

    Depositor

    William Hahn, David Root

    Available Since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only