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Plasmid#224376PurposeExpress NDK protein for his-tagged purificationDepositorInsertNDK (ndk E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)
Plasmid#225755PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_NKX2-5
Plasmid#221479PurposeGateway cloning of NKX2-5 overexpressionDepositorInsertNKX2-5 (NKX2-5 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAT10
Plasmid#204293PurposeFor marking CRISPR HDR integrant with DsRed expressed in flight muscles.DepositorInsertMHC-DsRed
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225241PurposeMammalian expression of iPASnFR-H in the cytosolDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDN
Plasmid#162450PurposeYeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsAga2p, HA, myc tags, and eUnaG2 fluorescent prote…ExpressionYeastMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
ttAtCas12a+int
Plasmid#182384PurposeGives very high editing efficiency in barley. Also works in Brassica oleraceaDepositorInsertLbCas12a coding sequence with D156R and Arabidopsis introns
UseCRISPRTagsExpressionMutationD156R + 8 Arabidopsis introns addedPromoterNoneAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5
Plasmid#225244PurposeMammalian expression of iPASnFR-L fused to Rab5DepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-OTI-TCRB_p2_TCRA
Plasmid#131034PurposeExpresses OT-I TCRB linked to TCRA with the P2A self-cleaving peptideDepositorArticleUseTagsExpressionMammalianMutationPromoterCMV/T7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)
Plasmid#225754PurposeEntry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detectionDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cox8-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225239PurposeMammalian expression of iPASnFR-H in mitochondriaDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSKI
Plasmid#232484PurposepSKI can be used for single-copy transgene expression within the Universal SKI System or for extrachromosomal arrays. It contains an MCS with 47 single cuts and two synthetic 900 bp homology arms.DepositorInsertpSKI
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbOcIgG-DddA11_NT
Plasmid#232441PurposeAnti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)DepositorInsertnbOcIgG-DddA11_N
UseTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-nbMmKappa-DddA11_NT
Plasmid#232442PurposeAnti-mouse kappa chain specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)DepositorInsertnbMmKappa-DddA11_NT
UseTagsExpressionMutationPromoterAvailable sinceFeb. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 VDAC1-TC
Plasmid#211733PurposeExpresses tetracysteine tagged VDAC1 in mammalian cells. TC-tag is attached to the C-terminus of VDAC1.DepositorInsertVoltage-dependent anion channel 1 (VDAC1 )
UseTagsTetracysteineExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP
Plasmid#218633PurposeProximity biotinylation of msNLRP3DepositorInsertNLRP3 (Nlrp3 Mouse)
UseLentiviralTagsAPEX2-3xFLAGExpressionMammalianMutationPromoterUBCAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJMH143
Plasmid#186646PurposeaadA.co-TaadA-PJ23117-eYFP.co between homology arms for pilADepositorArticleInsertaadA.co-TaadA-PJ23117-eYFP.co
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PTBP1-Halo
Plasmid#175302PurposeProduction of HaloTag fusion proteinDepositorInsertPTBP1 (PTBP1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL0_62 [sfYFP]
Plasmid#198974PurposeLevel 0 partDepositorInsertsfYFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 21, 2023AvailabilityAcademic Institutions and Nonprofits only