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pML2(+)BF

Plasmid

#108334

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (+) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(+)WR

Plasmid

#108335

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (+) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BF

Plasmid

#108338

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with forward orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)WR

Plasmid

#108339

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for AarI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pML2(−)BR

Plasmid

#108340

Purpose

MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

Depositor

Andrew Muscroft-Taylor

Article

van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB412

Plasmid

#68677

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB232

Plasmid

#68623

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB432

Plasmid

#68697

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Expression

Plant

Promoter

MpHSP17.8A1 promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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p201N 1514

Plasmid

#47025

Purpose

Contains soybean miRNA miR1514 recognition sequence to produce siRNAs from 3' target sequences and induce RNA silencing

Depositor

Wayne Parrott

Article

Jacobs et al Plant Biotechnol J. 2015 Mar 27. doi: 10

Inserts

gma-miR1514 recognition sequence
nptII selectable marker

Use

RNAi

Promoter

GmUbi and StUbi-3

Available Since

Aug. 30, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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ADEPT-pTarget-Msp4

Plasmid

#238037

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp1

Plasmid

#238034

Purpose

Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp2

Plasmid

#238035

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp3

Plasmid

#238036

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp6

Plasmid

#238039

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp5

Plasmid

#238038

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Available Since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM_ΔndhD2.km

Plasmid

#185532

Purpose

a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

nptII gene flanked by ndhD2 upstream and downstream regions

Use

Synthetic Biology

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Article

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA324

Plasmid

#214741

Purpose

Low copy glmS-nadE plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(8455)-glmS-RBS(5392)-nadE

Expression

Bacterial

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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pSV015

Plasmid

#214738

Purpose

Low copy thyA-infA plasmid containing kanamycin as antibiotic resistance gene (ARG) for validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23108-RBS(7504)-thyA-RBS(4427)-infA

Expression

Bacterial

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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