Addgene

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pRO217

Plasmid

#233807

Purpose

MoClo-YTK; type3; rtTA systems; rtTA-v16

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

rtTA-v16

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO174

Plasmid

#233809

Purpose

MoClo-YTK; type3A; ABA systems; Phlf-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Phlf-NES

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO271

Plasmid

#233813

Purpose

MoClo-YTK; type3A; Auxin systems; AmtR-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

AmtR-NES

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN050

Plasmid

#233815

Purpose

MoClo-YTK; type3B; Auxin systems; TIR1-U1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

TIR1-U1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN083

Plasmid

#233816

Purpose

MoClo-YTK; type3B; Auxin systems; TIR1-U2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

TIR1-U2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAL009

Plasmid

#233817

Purpose

MoClo-YTK; type3B; NS3 systems; NS3-V1

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3-V1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN018

Plasmid

#233818

Purpose

MoClo-YTK; type3B; NS3 systems; NS3-V2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3-V2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN080

Plasmid

#233820

Purpose

MoClo-YTK; type3B; NS3 systems; Linker-NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Linker-NS3a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN062

Plasmid

#233822

Purpose

MoClo-YTK; type3A; NS3 systems; LexA-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

LexA-NES

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO176

Plasmid

#233824

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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ClhN-p500-Erg6-V5-APEX2-URA

Plasmid

#207070

Purpose

Expressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Erg6

Use

Tags

V5-APEX2

Expression

Yeast

Mutation

Promoter

pERG6

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA

Plasmid

#207064

Purpose

Expressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Use

Tags

4xV5-APEX2 and GFP

Expression

Yeast

Mutation

Promoter

pADH3

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-OM14-V5-APEX2-Ura

Plasmid

#207073

Purpose

Expressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

OM14

Use

Tags

V5-APEX2

Expression

Yeast

Mutation

Promoter

pOM14

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-V5-APEX2-Ura

Plasmid

#207061

Purpose

Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Use

Tags

V5-APEX2

Expression

Yeast

Mutation

Promoter

pCOX4

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-p1k-Cox4-GFP-APEX2-Ura

Plasmid

#207062

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Use

Tags

GFP-APEX2

Expression

Yeast

Mutation

Promoter

pCOX4

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pADH3-Snf7-4V5-APEX2-URA

Plasmid

#207065

Purpose

Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Snf7

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pADH3

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pERG6-Pex3-4V5-APEX2-URA

Plasmid

#207068

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pERG6

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pLSP1-Pex3-4V5-APEX2-URA

Plasmid

#207069

Purpose

Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Pex3

Use

Tags

4xV5-APEX2

Expression

Yeast

Mutation

Promoter

pLSP1

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Emc1-GFP-APEX2-Ura

Plasmid

#207074

Purpose

Expressing Emc1-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Emc1

Use

Tags

GFP-APEX2

Expression

Yeast

Mutation

Promoter

pTPI1

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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ClhN-pTPI1-Cox4-GFP-APEX2-Ura

Plasmid

#207075

Purpose

Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

Depositor

Zhiping Xie

Article

Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

Insert

Cox4

Use

Tags

GFP-APEX2

Expression

Yeast

Mutation

Promoter

pTPI1

Available since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge