We narrowed to 13,392 results for: mpl

1. AAV 9P31-hSyn-Venus-P2A-Tau-RING

   Plasmid

   #233693

   Purpose

   AAV expression of Venus-P2A-Tau-RING from hSyn promoter

   Depositor

   Leo James

   Article

   Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

   Insert

   Venus-P2A-Tau-RING
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

   Plasmid

   #214950

   Purpose

   Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
   VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
   

   Use

   Tags

   6xHis, Amino half of venus BIFC fragment, Carboxy…

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-F-box:gs10:FRB:HA

   Plasmid

   #228531

   Purpose

   F-box domain of human Skp2 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   F-box:gs10:FRB:HA (SKP2 Human, Synthetic)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-myc:Rbx1:gs10:FRB:HA

   Plasmid

   #228534

   Purpose

   Human Rbx1 protein in fusion with FRB domain through a flexible glycine-serine linker.

   Depositor

   Roman Jerala

   Article

   Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

   Insert

   myc:Rbx1:gs10:FRB:HA (RBX1 Human, Synthetic)
   

   Use

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCA528 IST1 (322-366)

   Plasmid

   #193038

   Purpose

   Bacterial expression plasmid for IST1 (322-366), the minimal construct with full binding to CAPN7 MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 322-366

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-CAPN7-OSF

   Plasmid

   #193041

   Purpose

   Mammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   OSF (One-Strep-FLAG)

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta-actin

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCA528 CAPN7 L61D (1-165)

   Plasmid

   #193033

   Purpose

   Bacterial expression plasmid for CAPN7 (1-165) tandem MIT domains with L61D mutation

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L61D, Residues 1-165

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-C-Solo-mScarlet-MAPRE1

   Plasmid

   #227323

   Purpose

   Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. qTAG-C-Solo-mStayGold-EZR

   Plasmid

   #227294

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   EZR Homology Arms flanking a mStayGold Tag (EZR Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-Solo-mStayGold-H3C2

    Plasmid

    #227334

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a mStayGold Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-miRFP670nano3-H3C2

    Plasmid

    #227335

    Purpose

    Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. qTAG-C-Solo-mStayGold-MAPRE1

    Plasmid

    #227324

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAPRE1 Homology Arms flanking a mStayGold Tag (MAPRE1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Puro-mStayGold-TUBA1B

    Plasmid

    #227321

    Purpose

    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a Puro-2A-mStayGold Cassette (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. qTAG-N-Solo-mStayGold-MAP4

    Plasmid

    #227296

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    MAP4 Homology Arms flanking a mStayGold Tag (MAP4 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. qTAG-C-Solo-mStayGold-CEP192

    Plasmid

    #227289

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Promoterless

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA.5.1-FHS-TNRC6A-SD-S1332D/S1346D/S1616D/S1691D

    Plasmid

    #215910

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332D/S1346D/S1616D/S1691D mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332D, S1346D, S1616D, S169…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA.5.1-FHS-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

    Plasmid

    #215911

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    Flag-HA-SBP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A, S1616A, S169…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1-myc-GFP-TNRC6A-SD-S1332A/S1346A/S1616A/S1691A

    Plasmid

    #215905

    Purpose

    Expression of TNRC6A-silencing domain (SD) with S1332A/S1346A/S1616A/S1691A mutation

    Depositor

    Kumiko Ui-Tei

    Article

    Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    myc-GFP

    Expression

    Mammalian

    Mutation

    contain aa1224-1709, S1332A, S1346A, S1616A, S169…

    Promoter

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

    Plasmid

    #223146

    Purpose

    Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    NIPP1 (PPP1R8 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 143-224

    Promoter

    EF1a

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. CCM2(FL)_pET49b

    Plasmid

    #224066

    Purpose

    Bacterial expression of full-length CCM2 fused to an N-terminal GST. HRV-3C cleavage site

    Depositor

    Ben Goult

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    ccm2 (CCM2 Human)
    

    Use

    Tags

    GST; 6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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