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pDONR223_RBM10_p.Y580F

Plasmid

#82834

Purpose

Gateway Donor vector containing RBM10 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

Y580F

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MAP3K1 gRNA (BRDN0001147696)

Plasmid

#77825

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAP3K1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MAP3K1 (MAP3K1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-zFucci-pA-exorh-GFP (JDW 1489)

Plasmid

#242593

Purpose

A Tol2 expression vector containing the zebrafish Ubi promoter driving zFUCCI to label cell cycle state. Exorh-EGFP in the backbone to label pineal cells.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGeminin, mCherry-zCdt1

Use

Zebrafish expressio

Promoter

zebrafish Ubi

Available Since

April 8, 2026

Availability

Academic Institutions and Nonprofits only

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pTol2-Crestin-zFUCCI-IRES-EGFP-caax (JDW 1516)

Plasmid

#242601

Purpose

A Tol2 expression vector containing the Crestin enhancer driving FUCCI to label cell cycle in neural crest cells followed by an IRES-EGFP-caax to label all neural crest cells green.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mCerulean-zGEM-P2A-mCherry-zCdt1

Use

Zebrafish expression

Promoter

Crestin enhancer

Available Since

April 7, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682A_K686A_K694_K709A (4KA) POLH

Plasmid

#246406

Purpose

Expresses K682A_K686A_K694_K709A POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A_K686A_K694_K709A. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_ NLS~K682R_K686R_K69R_K709R (4KR) POLH

Plasmid

#246407

Purpose

Expresses K682R_K686R_K69R_K709R POLH with an N-terminal NLS~FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682R_K686R_K69R_K709R. Coding sequence has …

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG NEDD8

Plasmid

#221860

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG NEDD8 in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG NEDD8

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_WT POLH_ΔGG Ubiquitin

Plasmid

#221861

Purpose

Expresses FLAG-tagged WT POLH fused to ΔGG ubiquitin in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG and ΔGG Ubiquitin

Expression

Mammalian

Mutation

Coding sequence has been optimised for expression…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K709A POLH

Plasmid

#221864

Purpose

Expresses POLH mutated at K682A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A + K709A. Coding sequence has been opti…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_K682A_K686A_K694A_K709A POLH

Plasmid

#221865

Purpose

Expresses POLH mutated at K682A, K686A, K694A and K709A with a FLAG tag in mammalian cells

Depositor

Roger Woodgate

Article

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

FLAG

Expression

Mammalian

Mutation

POLH K682A, K686A, K694A + K709A. Coding sequence…

Promoter

CMV

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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Neo-2/15-TGFa-SPMn-SpyTag003

Plasmid

#246650

Purpose

Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)

Depositor

Mark Howarth

Article

Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021

Insert

Neo-2/15-TGFa-SPMn-SpyTag003

Tags

Signal peptide and SpyTag003

Expression

Mammalian

Mutation

Construct contains the tPA signal peptide, Neo2/…

Promoter

CMV promoter

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pSPcas9(BB)-2A-Puro V2.0 sgCHMP2B-2 aauucccaaaugaagauggc

Plasmid

#231998

Purpose

Expression of an sgRNA targeting CHMP2B, Cas9, and a PuroR marker

Depositor

Franz-Ulrich Hartl

Article

Sitron et al Mol Cell. 2025 Sep 18;85(18):3505-3523.e

Insert

CHMP2B-targeted sgRNA (CHMP2B Human)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only
pENTR2b-ITGB1(YYFF)

Plasmid

#215451

Purpose

Gateway entry vector with integrin beta1 NPxY mutant (YYFF)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Use

Gateway entry vector

Mutation

Mutations in the NPxY sites Y783F, Y795F; Silent …

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF3379

Plasmid

#144855

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ACTL6A (ACTL6A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

Plasmid

#229824

Purpose

A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE

Plasmid

#226382

Purpose

Expresses tau propagation ORF with V5 tag on 8D2Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

8D2Q.Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE

Plasmid

#226383

Purpose

Expresses tau propagation ORF with V5 tag on 20D3Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

20D3Q, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE

Plasmid

#226384

Purpose

Expresses tau propagation ORF with V5 tag on 8A2R mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

8A2R, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE

Plasmid

#226385

Purpose

Expresses tau propagation ORF with V5 tag on 20A3R mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

20A3R, Tau2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-).TagRFP.T-2A-V5-K281Q.Tau2N4R-WPRE

Plasmid

#226386

Purpose

Expresses tau propagation ORF with V5 tag on K281Q mutant 2N4R tau

Depositor

Bradley Hyman

Article

Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

Insert

Tau2N4R (MAPT synthetic, Human)

Tags

TagRFP.T.2A.V5

Expression

Mammalian

Mutation

K281Q, 2N4R

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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