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pSMART-HCKan-yibDp-VHH-JM3

Plasmid

#202477

Purpose

Expresses the nanobody JM3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody JM3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-J3

Plasmid

#202478

Purpose

Expresses the nanobody J3 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody J3

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-m36.4

Plasmid

#202479

Purpose

Expresses the nanobody m36.4 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody m36.4

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re5D06

Plasmid

#202480

Purpose

Expresses the nanobody Re5D06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re5D06

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-VHH-Re9F06

Plasmid

#202481

Purpose

Expresses the nanobody Re9F06 after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

nanobody Re9F06

Tags

6X-His

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pSMART-HCKan-yibDp-roGFP2

Plasmid

#202462

Purpose

Expresses redox sensitive GFP (roGFP) after phosphate depletion

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

reduction-oxidation sensitive green fluorescent protein

Expression

Bacterial

Promoter

yibDp

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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p8270 LentiCRISPR v2 hygro sgSIGIRR-3

Plasmid

#193979

Purpose

Expression of spCas9 and sgRNA targeting SIGIRR

Depositor

Elizabeth White

Article

Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

Insert

spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)

Use

Lentiviral

Available Since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pNMTMA_MBP-Z-Cav1-His6

Plasmid

#223701

Purpose

To produce z-spheres (caveospheres containing the anti-IgG Z-domain of protein A), Parton Lab clone LCU

Depositor

Rob Parton

Article

T. D. et al bioRxiv

Insert

MBP-Z-Cav1-His6

Expression

Bacterial

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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TL1 alphaDdCBE T2 (DddA6-N)

Plasmid

#221995

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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ATP6 alphaDdCBE G2

Plasmid

#221959

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A, See depositor comments

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-GAC site)

Plasmid

#170149

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(GAC)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-CDK8

Plasmid

#222609

Purpose

Expression of MAC3-tagged CDK8

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

CDK8 (CDK8 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 6, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RepairTemplate_AAVS1-OsTIR1-F74G-SNAP

Plasmid

#217665

Purpose

Repair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locus

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

OsTir1 F74G-SNAP-Blast-AAVS1

Tags

SNAP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-HsTtyh1-TwinStrep-His

Plasmid

#211332

Purpose

Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Ttyh1 (W417R)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Tweety homology protein 1

Use

Lentiviral

Tags

TwinStrep, 10xHis

Expression

Mammalian

Mutation

W417R

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2-HsProm1s1-StayGold

Plasmid

#210821

Purpose

Mammalian overexpression vector for C-terminally StayGold tagged human Prom1s1 (WT)

Depositor

Luke Chao

Article

Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

Insert

Prominin 1 (PROM1 Human)

Tags

StayGold

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTP489

Plasmid

#216366

Purpose

xNup155-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup155-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC263

Plasmid

#216367

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC100

Plasmid

#216369

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC99

Plasmid

#216370

Purpose

xhNup155-Nb3i Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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