We narrowed to 20,110 results for: Cre

1. pLib6.4-Cquin_596

   Plasmid

   #176655

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMALp2x-SCO5461

   Plasmid

   #42507

   Depositor

   Koji Okamoto

   Article

   Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

   Insert

   guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))
   

   Tags

   maltose-binding protein

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pSL1180-HR-PUbEGFP-NLS

   Plasmid

   #176681

   Purpose

   Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   EGFP
   

   Expression

   Insect

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. p35S::mCh-HA-P2A-lifeact-mCh

   Plasmid

   #135234

   Purpose

   constitutive expression of 2 mCherry molecules: one tagged with HA and another that binds to actin (control plasmid)

   Depositor

   Armin Djamei

   Article

   Alcantara et al Plant Methods. 2020 Jan 21;16:4. doi: 10

   Insert

   mCh-HA-P2A-lifeact-mCh
   

   Tags

   HA, lifeact, and p2a

   Expression

   Plant

   Promoter

   35S

   Available Since

   Jan. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. actc1b-mKate2-rab5aa

   Plasmid

   #109648

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to rab5aa

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab5aa (rab5aa Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. actc1b-mKate2-rab5ab

   Plasmid

   #109649

   Purpose

   Muscle specific zebrafish expression of mKate2 fused to rab5ab

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   rab5ab (rab5ab Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   mKate2

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR-v1-sgMPC2_7

   Plasmid

   #163457

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 7 targeting GPT2 (MPC2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiCRISPR-v1-sgMPC2_9

   Plasmid

   #163458

   Purpose

   lentiviral vector expressing Cas9 and an sgRNA targeting MPC2

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   sgRNA 9 targeting GPT2 (MPC2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGWB527

   Plasmid

   #74869

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. HB901: pMVP (L3-L2) polyA

    Plasmid

    #121746

    Purpose

    pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    Polyadenylation signal (polyA)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-sgKmt2d-sg3

    Plasmid

    #192167

    Purpose

    Lenti-sgKmt2d-sg3

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    Lentiviral

    Mutation

    NA

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. KHBD00322

    Plasmid

    #34447

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8591 (CTCF Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pGWB525

    Plasmid

    #74867

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with T7 (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. KHBD00311

    Plasmid

    #34436

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG9786 (hb Fly)
    

    Use

    Gateway donor vector

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16

    Plasmid

    #89872

    Purpose

    expresses CB1R-iTango component in mammalian cells

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    CB1R-V2tail-TevN-BLITz1-TetR-VP16
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC193

    Plasmid

    #118903

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP, cleavable with 3C and monomeric GFP and C-terminal TwinStrep, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease; mGFP and TwinStr…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSCV-puro-TTF-1-HDD

    Plasmid

    #74991

    Purpose

    Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    TTF-1 HDD mutant (NKX2-1 Human)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    HDD = homeodomain deleted

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPA-RL-FL

    Plasmid

    #78863

    Purpose

    DULIP Tandem Construct used for data normalization

    Depositor

    Erich Wanker

    Article

    Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

    Insert

    Firefly
    

    Use

    Expression vector

    Tags

    Protein A, Renilla luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Str-KDEL_ManII-SBP-tagBFP

    Plasmid

    #65254

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. IF701: pMVP (L5-L4) APEX2

    Plasmid

    #121725

    Purpose

    pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    APEX2 (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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