We narrowed to 59,446 results for: cloning

1. pEN41A-T38S38

   Plasmid

   #49524

   Purpose

   Ampicillin resistant; entry clone encoding reverse TetR (tetR38) and contains attL4 and attL1 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   constitutive TetR38
   

   Promoter

   Ptb38

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN12A-P750

   Plasmid

   #49525

   Purpose

   Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750
   

   Promoter

   P750

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDA_791

   Plasmid

   #216079

   Purpose

   Minimal plasmid; contains only ori and ampR, destination vector

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Destination

   Expression

   Bacterial

   Available Since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AA163

   Plasmid

   #216061

   Purpose

   Fragmid fragment: (2A Selection) Stop only

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Stop
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPuroR-MCS-LexA-VP16-MiniWhite

   Plasmid

   #165898

   Purpose

   Puromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloning

   Depositor

   Koen Venken

   Article

   Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

   Insert

   Selectable Empty Enhancer Driver
   

   Use

   Synthetic Biology

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. AA082

   Plasmid

   #215997

   Purpose

   Fragmid fragment: (Selection) fluorescent reporter

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Has Service

   Cloning Grade DNA

   Insert

   Start; mTagBFP2_v1.1
   

   Use

   Fragment

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR223-BKRF3

   Plasmid

   #195790

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BKRF3

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BKRF3
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR223-BGLF2

   Plasmid

   #195800

   Purpose

   Gateway Entry Clone of Epstein-Barr virus BGLF2

   Depositor

   Eric Johannsen

   Article

   Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   BGLF2
   

   Use

   Gateway entry vector

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. 8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA

   Plasmid

   #124546

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
   

   Expression

   Mammalian

   Mutation

   WT, WT

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. AA176

    Plasmid

    #216063

    Purpose

    Fragmid fragment: (2A Selection) mCherry

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    P2A_v1.1; mCherry_v1.1; Stop
    

    Use

    Fragment

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHSG1C3

    Plasmid

    #164423

    Purpose

    single guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.

    Depositor

    Xiao Wang

    Article

    Standage-Beier et al ACS Synth Biol. 2021 Feb 19;10(2):422-42

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. DDX5 D248N V5

    Plasmid

    #199577

    Purpose

    Expresses helicase dead DDX5 cloned into pLenti puro

    Depositor

    Michael Kastan

    Article

    McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

    Insert

    DDX5 (DDX5 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Mutation

    D248N

    Promoter

    CMV

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3269 pBS Bcl-2

    Plasmid

    #8764

    Depositor

    Stanley Korsmeyer

    Article

    Silverman et al Proc Natl Acad Sci U S A 1990 Dec;87(24)

    Insert

    Bcl-2 (Bcl2 Mouse)
    

    Expression

    Bacterial

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pQTEV-COPE

    Plasmid

    #31585

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    coatomer protein complex, subunit epsilon (COPE Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. UKNP4.3.2

    Plasmid

    #98270

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. UKNP3.1.1

    Plasmid

    #98214

    Purpose

    Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

    Depositor

    Jonathan Ball

    Article

    Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

    Insert

    HCV E1E2
    

    Expression

    Mammalian

    Available Since

    Aug. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5/FRT/TO DNAJA1

    Plasmid

    #19464

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJA1 (DNAJA1 Human)
    

    Expression

    Mammalian

    Mutation

    D244G

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19-TSV (linearized in VP2)

    Plasmid

    #70032

    Purpose

    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region

    Depositor

    Mariet Feltkamp

    Article

    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.

    Insert

    TSV full genome, linearized in VP2 gene
    

    Use

    Cloning vector

    Expression

    Bacterial

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO DNAJA2

    Plasmid

    #19465

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ subfamily A member 2 (DNAJA2 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 10, 2008

    Availability

    Academic Institutions and Nonprofits only