We narrowed to 14,728 results for: EGF

1. GFP-MYO10-BioID

   Plasmid

   #194858

   Purpose

   Express MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   MYO10 (MYO10 Human)
   

   Tags

   BioID and EGFP

   Expression

   Mammalian

   Mutation

   MyoX contains a Q680R variant compared to the NCB…

   Promoter

   CMV

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-DeAct-SpvB

   Plasmid

   #89446

   Purpose

   Expresses EGFP-SpvB (Salmonella SpvB 375-591) in mammalian cells

   Depositor

   Brad Zuchero

   Article

   Harterink et al Nat Methods. 2017 Apr 10. doi: 10.1038/n

   Insert

   DeAct-SpvB (spvB Salmonella enterica)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Truncation spanning SpvB amino acids 375-591 (mon…

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GFP-AuroraB kd K106R

   Plasmid

   #108493

   Purpose

   expression of EGFP-AuroraB kinase dead (K106R)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Kinase death K106A

   Promoter

   CMV

   Available Since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST-CMV GFP-LC3B G120A

   Plasmid

   #123115

   Purpose

   Expresses EGFP-LC3B G120A in mammalian cells. Negative control fluorescent reporter, unable to localize to autophagosomes.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 120 to Alanine

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHRIG-AktDN

   Plasmid

   #53597

   Purpose

   lentiviral expression of dominant negative mouse Akt1 and EGFP

   Depositor

   Heng Zhao

   Article

   Xie et al J Cereb Blood Flow Metab. 2013 Dec;33(12

   Insert

   Akt1 (Akt1 Mouse)
   

   Use

   Lentiviral

   Tags

   HA and IRES-EGFP

   Expression

   Mammalian

   Mutation

   Dominant negative (K179M)

   Promoter

   CMV

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. miniCGBE1-VRQR (pBM1173)

   Plasmid

   #140255

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)_VRQR-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

   Plasmid

   #168107

   Purpose

   PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

   Depositor

   Erich Jarvis

   Article

   Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

   Insert

   Cas9-P2A-mCherry
   

   Use

   CRISPR and Cre/Lox

   Tags

   HA

   Promoter

   CAG

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Cx32R220stop

   Plasmid

   #78151

   Purpose

   Mammalian Expression of the first 219 amino acids of Connexin 32 and EGFP from a bicistronic mRNA

   Depositor

   Mario Bortolozzi

   Article

   Carrer et al Hum Mol Genet. 2017 Oct 25. pii: 4565496

   Insert

   Connexin 32 (GJB1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Only contains first 219 residues

   Promoter

   CMV

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

9. pDEST-CMV 3xFLAG-LC3A-GFP

   Plasmid

   #123106

   Purpose

   Expresses 3xFLAG-LC3A-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3A

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
   

   Tags

   3xFLAG and EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST-CMV 3xFLAG-GABARAPL1-GFP

    Plasmid

    #123112

    Purpose

    Expresses 3xFLAG-GABARAPL1-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL1

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LentiU6-LacZ-GFP-Puro (BB)

    Plasmid

    #170459

    Purpose

    For cloning gRNA block

    Depositor

    Yonglun Luo

    Article

    Xiang et al Nat Commun. 2021 May 28;12(1):3238. doi:

    Insert

    hU6-BB-LacZ and EF1A-EGFP-2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6 for gRNA and EF1A for EGFP-2A-Puro

    Available Since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Promoter

    CAG

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pssGFP-hCRT

    Plasmid

    #80022

    Purpose

    Mammalian expression of signal peptide-EGFP-humanCRT

    Depositor

    Chinten James Lim

    Article

    Liu et al Cell Death Dis. 2016 Jun 16;7:e2268. doi

    Insert

    CRT (CALR Human)
    

    Tags

    EGFP and signal peptide

    Expression

    Mammalian

    Mutation

    signal peptide 17aa added before GFP-CRT, will ta…

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pDEST-CMV 3xFLAG-GABARAPL2-GFP

    Plasmid

    #123113

    Purpose

    Expresses 3xFLAG-GABARAPL2-EGFP in mammalian cells, for monitoring ATG4 activity towards GABARAPL2

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHRIG-PRAS40

    Plasmid

    #53599

    Purpose

    lentiviral expression of constitutively active PRAS40 and EGFP

    Depositor

    Heng Zhao

    Article

    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1

    Insert

    PRAS40 (AKT1S1 Human)
    

    Use

    Lentiviral

    Tags

    IRES-EGFP and myc

    Expression

    Mammalian

    Mutation

    constitutively active (myristoylated version)

    Promoter

    CMV

    Available Since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

16. SECURE BE4max(R33A) (pBM608)

    Plasmid

    #140251

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    BE4max(R33A)
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, D10A in Cas9

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mILK-R255A/R349A-EFGP

    Plasmid

    #176899

    Purpose

    mouse ILK with R255A and R349A point mutations cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Arginine 255 to Alanine and Arginine 349 to Alani…

    Promoter

    CNV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST-CMV GFP-LC3B G120

    Plasmid

    #123116

    Purpose

    Expresses EGFP-LC3B G120 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted amino acids 121-125. Stop codon after G12…

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GFP-AuroraB L154G/H250Y

    Plasmid

    #108492

    Purpose

    expression of EGFP-AuroraB Analog sensitive (L154G/H250Y)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Analog sensitive L154G/H250Y

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hFLT1 delta 754-763

    Plasmid

    #86035

    Purpose

    human VEGFR1 with a 10 amino acid deletion in EC close to TM domain

    Depositor

    Christie Thomas

    Article

    Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11.

    Insert

    human Flt1 delta754-763 aa (FLT1 Human)
    

    Tags

    FLAG, HA, and Myc

    Expression

    Mammalian

    Mutation

    delta 754-763

    Promoter

    CMV

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge