We narrowed to 309,303 results for: addgene

1. MAP3K7CL_pCSdest

   Plasmid

   #53808

   Depositor

   Roger Reeves

   Article

   Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

   Insert

   MAP3K7CL (C21orf7 Human)
   

   Expression

   Mammalian

   Promoter

   SP6 and CMV

   Available Since

   March 27, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

2. pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

   Plasmid

   #107728

   Purpose

   Knock out of EB1 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

   Plasmid

   #133790

   Purpose

   CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   human codon optimized NmeCas9
   

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CAG

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBT259_(pRosa26-G-tTA2)

   Plasmid

   #36878

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   GFP
   tTA2
   beta-globin intron
   Neo
   diphteria toxin A
   

   Expression

   Mammalian

   Mutation

   deleted nucleotides after nucleotide 274, inserti…

   Promoter

   CAG (chicken beta actin promoter and CMV enhancer…

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Lenti_CRISPRi_sgNeg2

   Plasmid

   #166999

   Purpose

   Lentiviral expression of negative control sgRNA for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   Negative control sgRNA 2
   

   Use

   CRISPR and Lentiviral

   Promoter

   U6

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FH95pUp95bGW (B5)

   Plasmid

   #74014

   Purpose

   lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion and Psd95 shRNA

   Depositor

   Robert Malenka, Oliver Schluter, Weifeng Xu

   Article

   Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

   Insert

   Psd95 shRNA (Dlg4 Rat)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   H1

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOTTC813 - pAAV-SYN1-CRTsigpep-GCaMP3-KDEL

   Plasmid

   #63886

   Purpose

   An AAV packaging vector that expresses ER-retained GCaMP3 under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized GCaMP3(wt)
   

   Use

   AAV

   Promoter

   human SYN1

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Zebrafish-gRNA-0001

   Plasmid

   #42241

   Purpose

   gRNA targeted to zebrafish gene apoea

   Depositor

   Keith Joung

   Article

   Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

   Insert

   gRNA-apoea (apoea Zebrafish)
   

   Use

   CRISPR; Zebrafish expression

   Promoter

   T7

   Available Since

   Jan. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

9. gh176

   Plasmid

   #106837

   Purpose

   expression of gRNA targeting HAS2

   Depositor

   Eric-Paul Bennett

   Article

   Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

   Insert

   HAS2 (HAS2 Human)
   

   Expression

   Mammalian

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. gh175

    Plasmid

    #106836

    Purpose

    expression of gRNA targeting HAS1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    HAS1 (HAS1 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. gh177

    Plasmid

    #106827

    Purpose

    expression of gRNA targeting HAS3

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    HAS3 (HAS3 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PMYT1_HUMAN_D0

    Plasmid

    #79687

    Purpose

    This plasmid encodes the kinase domain of PMYT1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    PMYT1
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. gh267

    Plasmid

    #106866

    Purpose

    expression of gRNA targeting ALG13

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ALG13 (ALG13 Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe puro human Frataxin

    Plasmid

    #11505

    Depositor

    Ronald Kahn

    Article

    Ristow et al Proc Natl Acad Sci U S A. 2000 Oct 24. 9

    Insert

    Frataxin (FXN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    March 3, 2006

    Availability

    Academic Institutions and Nonprofits only

15. FB002

    Plasmid

    #119676

    Purpose

    Terminator tub from Neurospora crassa domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Terminator tub
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FB008

    Plasmid

    #119706

    Purpose

    Terminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Terminator trpC
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFETCh_ARID4B

    Plasmid

    #101072

    Purpose

    Donor vector for 3' FLAG tag of human ARID4B

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    ARID4B homology arms (ARID4B Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

19. JARID2-KO-gRNA-2

    Plasmid

    #131327

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

20. pIRES-EGFP-TPD52

    Plasmid

    #172460

    Purpose

    Expression of untagged Tumor Protein D52 (TPD52) and GFP

    Depositor

    Stephen Royle

    Article

    Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    TPD52 (TPD52 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge