We narrowed to 309,303 results for: addgene

1. pX458-sgRNA_Ago1_3

   Plasmid

   #73535

   Purpose

   Expresses SpCas9, GFP, and sgRNA targeting Ago1

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   AGO1
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

2. 8xCTS1_deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124544

   Purpose

   Cloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55A-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55A, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 8xCTS1_delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124540

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   delta-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro_SV40polyA

   Plasmid

   #124538

   Purpose

   Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

   Depositor

   Tudor Fulga

   Article

   Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

   Insert

   deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_delta-hutRNApro
   

   Expression

   Mammalian

   Mutation

   d11-41 C55G, d11-41

   Promoter

   Minimal ADE

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PRPF4B gRNA (BRDN0001146164)

   Plasmid

   #76428

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human PRPF4B

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   PRPF4B
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 49535-sgFMR1_E17A

   Plasmid

   #157782

   Purpose

   Expresses a sgRNA targeting stop codon of human FMR1 gene

   Depositor

   Xinyu Zhao

   Article

   Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

   Insert

   FMR1 (FMR1 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   March 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. RCIscript-GoldyTALEN-ponzr1L

   Plasmid

   #42654

   Depositor

   Stephen Ekker

   Article

   Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

   Insert

   ponzr1 left pTAL scaffold
   

   Use

   Mrna transcription vector

   Promoter

   T3

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. LCV2 V-4

   Plasmid

   #84807

   Purpose

   Cas9 with sgRNA targeting Exon 1 of VEGF-A

   Depositor

   Glenn Yiu

   Article

   Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

   Insert

   gRNA targeting VEGF-A (VEGFA Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSN67

   Plasmid

   #100124

   Purpose

   expresses putative KLF4 DBD with a 6xHis tag

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCherry-P2A-putative KLF4 DBD with a 6xHis tag (KLF4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Insert corresponds to the DBD of KLF4

   Available Since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. OVA-miSFIT-19-G

    Plasmid

    #124676

    Purpose

    Tuning expression of cytosolic Ovalbumin coupled to EGFP

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    Cyto-Ovalbumin
    

    Expression

    Mammalian

    Mutation

    Truncated OVA for cytoplasmic expression

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. gh6

    Plasmid

    #106690

    Purpose

    expression of gRNA targeting GALNT6

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    GALNT6 (GALNT6 Human)
    

    Expression

    Mammalian

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. LCV2 V-5

    Plasmid

    #84808

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiGuide-Puro-CTRLg2 (CG510)

    Plasmid

    #139456

    Purpose

    Lentiviral vector with non-targeting gRNA and puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    Non-targeting sgRNA inserted; resistance gene: puroR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFETCh_RERE

    Plasmid

    #101069

    Purpose

    Donor vector for 3' FLAG tag of human RERE

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RERE homology arms (RERE Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX458_RERE_1

    Plasmid

    #101073

    Purpose

    Encodes gRNA for 3' target of human RERE

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against RERE (RERE Human)
    

    Use

    CRISPR

    Available Since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CSK21_HUMAN_D0

    Plasmid

    #79707

    Purpose

    This plasmid encodes the kinase domain of CSK21. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    CSK21
    

    Tags

    His10-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pCNL-C1_Rab11a

    Plasmid

    #65706

    Purpose

    Expresses CNL-Rab11a in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Rab11a (Rab11a Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. ZAK gRNA (BRDN0001145178)

    Plasmid

    #75596

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAK
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. ZAK gRNA (BRDN0001146540)

    Plasmid

    #75597

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ZAK

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ZAK
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RCIscript-GoldyTALEN-ponzr1R

    Plasmid

    #42655

    Depositor

    Stephen Ekker

    Article

    Bedell et al Nature. 2012 Nov 1;491(7422):114-8. doi:

    Insert

    ponzr1 right pTAL scaffold
    

    Use

    Mrna transcription vector

    Promoter

    T3

    Available Since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge