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pRoTarget

Plasmid

#178774

Purpose

partial tRNA-Leugene from Rippkaea orientalis

Depositor

Chase Beisel

Article

Wimmer et al Mol Cell. 2022 Feb 16. pii: S1097-2765(2

Insert

partial tRNA-Leugene from Rippkaea orientalis

Expression

Bacterial

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046658

Plasmid

#149226

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046682

Plasmid

#149197

Purpose

Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046642

Plasmid

#149164

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046687

Plasmid

#149148

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDUET-ctCPR-trAMO

Plasmid

#20112

Depositor

Jay Keasling

Article

Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

Insert

p450 reductase and truncated amorphadiene oxidase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
KVS1-HA

Plasmid

#113061

Purpose

Expresses worm KVS-1 in mammalian cells

Depositor

Federico Sesti

Article

Cai et al Nat Neurosci. 2005 Nov;8(11):1503-9. doi

Insert

K+ voltage sensitive channel subunit 1 (kvs-1 Nematode)

Tags

HA

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4929

Plasmid

#126751

Purpose

Expresses pTRX2_5xUAS-ALD6-tADH1 in yeast from plasdmid pRS413

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

ALD6

Expression

Yeast

Promoter

pTRX2_5xUAS

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046182

Plasmid

#149019

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pTEF1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMiR-HPV16-E7/E1

Plasmid

#53703

Purpose

Luciferase reporter assay for microRNA binding on HPV16 E7/E1

Depositor

Edward Chan

Article

Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

Insert

HPV16-E7/E1

Use

Luciferase

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pGBW-m4046374

Plasmid

#149120

Purpose

Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046372

Plasmid

#149048

Purpose

Yeast Expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046521

Plasmid

#145473

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp11

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp11 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDS295

Plasmid

#34984

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

ePDZb

Expression

Yeast

Promoter

GAL1

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046387

Plasmid

#149005

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBSc + Shal

Plasmid

#16142

Depositor

Larry Salkoff

Article

Fawcett et al J Biol Chem. 2006 Oct 13. 281(41):30725-

Insert

Voltage-gated K+ channels (Y73B6BL.19 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only
pGBW-m4046170

Plasmid

#149119

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp10

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046415

Plasmid

#149035

Purpose

Yeast Expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 helicase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046246

Plasmid

#145553

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp7

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046231

Plasmid

#145451

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp8

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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