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Showing: 8121 - 8140 of 18384 results

  3. pET-FLAG-eSpCas9-plus

    Plasmid
    #126774
    Purpose
    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Insert
    eSpCas9-plus
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, MBP, and NLS
    Expression
    Bacterial
    Mutation
    K848A, R1060A; amino acids 1005-1013 replaced wit…
    Promoter
    T7
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126774-plasmid-map-sequence-id-410603

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  10. pChlr-2SE

    Plasmid
    #52144
    Purpose
    Encodes module 2 with amino acids 12S-16E for recognition of nt 7G
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 2
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pChlr-8CQ

    Plasmid
    #52171
    Purpose
    Encodes module 8 with amino acids 12C-16Q for recognition of nt 1A
    Depositor
    Huimin Zhao
    Article
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in module 8
    Available Since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pUASp YFP Rholike DN

    Plasmid
    #52240
    Purpose
    Drosophila expression of YFP-Rholike dominant negative mutant
    Depositor
    Matthew Scott
    Insert
    Rholike (RhoL Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    T25N; DN mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pCDNA3-hPXR-FL-T248E

    Plasmid
    #41617
    Depositor
    Sridhar Mani
    Insert
    hPXR-FL-T248E (NR1I2 Human)
    Expression
    Mammalian
    Mutation
    N10D and T248E
    Promoter
    CMV
    Available Since
    March 11, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pLenti4/V5-DEST-P85(R574fs)

    Plasmid
    #40227
    Depositor
    Lynda Chin
    Insert
    P85(R574fs) (PIK3R1 Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Introduced frame shift mutation at amino acid 574
    Promoter
    CMV
    Available Since
    Oct. 11, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 8121 - 8140 of 18384 results