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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Publication

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200)

Plasmid

#119857

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF1(100-200) (YTHDF1 100-200 subdomain)

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13b-GGS-NYTHDF1(100-200)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200)

Plasmid

#119856

Purpose

Cmv d0 dPspCas13b-GGS-NYTHDF2(100-200) (YTDHF2 100-200 subdomain)

Depositor

Bryan Dickinson

Publication

Rauch et al J Am Chem Soc. 2018 Sep 26;140(38):11974

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas13b-GGS-NYTHDF2(100-200)

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pYS1

Plasmid

#117488

Purpose

Generation of unmarked gene deletions in Mycobacterium smegmatis

Depositor

Eyal Gur

Publication

Shenkerman et al Gene. 2014 Jan 1;533(1):374-8. doi: 10.1

Insert

Che9c 60–61, sacB, aph

Use

Deletion generation

Promoter

Acetamide

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-NLS-actin

Plasmid

#118380

Purpose

Gateway compatible donor vector with nuclear localization signal (NLS) attached to WT actin.

Depositor

Maria Vartiainen

Publication

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Insert

actin beta (ACTB Human)

Use

Donor-plasmid

Mutation

NLS

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-TRAF1

Plasmid

#111199

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAF1 (TRAF1 Human)

Tags

EGFP-FLAG

Expression

Mammalian

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-150

Plasmid

#118532

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn5-153

Plasmid

#118535

Purpose

miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Use

Minitn5 delivery vector

Expression

Bacterial

Available since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE137

Plasmid

#118491

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Expression

Bacterial

Available since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pF709-pET28a-Hs-FL-ETFbeta-NHis wt

Plasmid

#85110

Purpose

expression of recombinant human full-length ETFbeta wt with N-terminal 6xHis-tag

Depositor

Pal Falnes

Publication

Malecki et al J Biol Chem. 2015 Jan 2;290(1):423-34. d

Insert

human full-length ETFbeta (ETFB Human)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE166

Plasmid

#118514

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mCardinal

Expression

Bacterial

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSG22-pBAD-TetR

Plasmid

#102451

Purpose

Expresses TetR protein in E. coli. The expression is controlled by a pBAD promoter.

Depositor

Richard Murray

Publication

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

TetR (tetR )

Expression

Bacterial

Promoter

pBAD

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-157

Plasmid

#118571

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mClover3

Use

Minitn7 delivery vector

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE141

Plasmid

#118493

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Expression

Bacterial

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pVimentin-Spot-Tag

Plasmid

#117059

Purpose

For mammalian expression of vimentin with C-terminal Spot-Tag

Depositor

Felix Hartlepp

Insert

vimentin (VIM Human)

Tags

Spot-Tag

Expression

Mammalian

Promoter

CMV

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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M3-PSCFP2

Plasmid

#114185

Purpose

studying clustering of M3 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Publication

Scarselli et al J Biol Chem. 2012 May 11;287(20):16768-8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Muscarin acetylcholine receptor 3 (CHRM3 Human)

Tags

3xHA and PS-CFP2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCOLA banana sensor sfGFP

Plasmid

#107367

Purpose

Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified banana DNA

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

banana-detecting toehold + sfGFP

Expression

Bacterial

Promoter

T7

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9-D10A

Plasmid

#61477

Purpose

based on pDe-CAS9, encodes Cas9 D10A nickase variant

Depositor

Holger Puchta

Publication

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9-D10A

Use

CRISPR

Expression

Plant

Mutation

catalytic Asp10 changed to Ala

Promoter

PcUbi4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR ARRET

Plasmid

#111384

Purpose

ARRET with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

Depositor

Klaus Holzmann

Insert

ARRET

Use

Gateway entry cloning

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR1a-tagRFP-KDEL

Plasmid

#114177

Purpose

Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

Depositor

Nicolas Demaurex

Publication

Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tagRFP-KDEL

Use

Gateway Cloning

Tags

KDEL retention signal and signal peptide

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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