We narrowed to 32,277 results for: uros

1. pNM1325

   Plasmid

   #197144

   Purpose

   CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

   Depositor

   Nozomu Yachie

   Article

   Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

   Insert

   SpCas9-PmCDA1-UGI
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   SpCas9(D10A)

   Promoter

   CAG

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GFP-IRES-hSNCA (S129D)

   Plasmid

   #185719

   Purpose

   AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ser 129 to Asp

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GFP-IRES-hSNCA (S129A)

   Plasmid

   #185718

   Purpose

   AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ser 129 to Ala

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GFP-IRES-mSNCA(1-95)

   Plasmid

   #185716

   Purpose

   AAV expression of GFP and C-terminal truncated (1-95 amino acid) mouse α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (Snca Mouse)
   

   Use

   AAV

   Mutation

   deleted AA 95-144

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF TV

   Plasmid

   #105196

   Purpose

   targeting vector to generate a Gucy1b2-IRES-nlsCre-IRES-taulacZ mouse strain.

   Depositor

   Peter Mombaerts

   Article

   Koike et al Neuron. 2021 Aug 4;109(15):2469-2484.e7.

   Insert

   Gucy1b2-IRES-nlsCre-IRES-taulacZ-FNF (Gucy1b2 Mouse)
   

   Use

   Mouse Targeting

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

6. FUS_LC_charge_RtoK

   Plasmid

   #139127

   Purpose

   express FUS LC with hnRNPA2-like charge patterning where all R are changed to K

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Expression

   Bacterial

   Mutation

   Add K and D to FUS LC so it has the same charge p…

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV-gRNA-Bcan-Ntrk1_4

   Plasmid

   #136413

   Purpose

   Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV-gRNA-Myb-Qk_1

   Plasmid

   #136415

   Purpose

   Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

   Depositor

   Massimo Squatrito

   Article

   Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

   Insert

   U6_sgRNA(Myb)_U6_sgRNA(Qk)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-Fyn-cGASΔN-HA

   Plasmid

   #130922

   Purpose

   Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   Fyn-ΔNcGAS
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   cGAS N terminus (aa1-159) replaced with the dual …

   Promoter

   CMV

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJet-CMV-CD52-bglpA

    Plasmid

    #89704

    Purpose

    Human CD52 expression plasmid with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD52-bglpA (CD52 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-PIG-N3FLAG-TAF12

    Plasmid

    #105593

    Purpose

    retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    silent mutations to make it resistant to the targ…

    Promoter

    MSCV-LTR

    Available Since

    May 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-PIG-N3FLAG-TAF12(50-130)

    Plasmid

    #105595

    Purpose

    retrovirally express mouse TAF12 HFD with 3*FLAG tag at N terminal, GFP marker and puro resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 (AA 50-130)- histone fold domain-HFD (Taf12 Mouse)
    

    Use

    Retroviral

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    truncation fragments containing aa (50-130), and …

    Promoter

    MSCV-LTR

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRKCG gRNA (BRDN0001147740)

    Plasmid

    #76898

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCG (PRKCG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PRKCG gRNA (BRDN0001146492)

    Plasmid

    #76899

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCG (PRKCG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PRKCG gRNA (BRDN0001146495)

    Plasmid

    #76900

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKCG

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKCG (PRKCG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PRKAR1B gRNA (BRDN0001146606)

    Plasmid

    #76838

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PRKAR1B gRNA (BRDN0001144765)

    Plasmid

    #76839

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PRKAR1B gRNA (BRDN0001147089)

    Plasmid

    #76840

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PRKAR1B

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PRKAR1B (PRKAR1B Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PANK2 gRNA (BRDN0001162474)

    Plasmid

    #76810

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PANK2

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PANK2 (PANK2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge