We narrowed to 24,139 results for: Spr

1. pLentiCRISPR v2 puro hGSDME gRNA2

   Plasmid

   #223520

   Purpose

   Knocking out hGSDME in human cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-CASPR2 [K67/25R]

   Plasmid

   #177446

   Purpose

   Mammalian Expression Plasmid of anti-CASPR2 (Human). Derived from hybridoma K67/25.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-CASPR2 (Homo sapiens) recombinant mouse monoclonal antibody (CNTNAP2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR v2-sgAMPK alpha2 clone1

   Plasmid

   #162122

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha2

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha2 (PRKAA2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

   Plasmid

   #218524

   Purpose

   sgRNA targeting human KIAA0141/DELE1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   DELE1 (DELE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

   Plasmid

   #218525

   Purpose

   sgRNA targeting human KIAA0141/DELE1

   Depositor

   Kivanc Birsoy

   Article

   Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

   Insert

   DELE1 (DELE1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMTP170 (pBAD33_CRISPR(Tn6900)-entry)

   Plasmid

   #164263

   Purpose

   Entry vector for cloning user-defined spacers in the Tn6900 CRISPR array.

   Depositor

   Joseph Peters

   Article

   Petassi et al Cell. 2020 Nov 30. pii: S0092-8674(20)31

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Arabinose

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCRISPR v2-Nr4a1 double gRNAs

   Plasmid

   #162791

   Purpose

   Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

   Depositor

   Matthew Steinhauser

   Article

   Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

   Insert

   Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. R777-E322 Hs.SPRED1-nostop

   Plasmid

   #70606

   Purpose

   Gateway ORF clone of human SPRED1 [NM_152594.2] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SPRED1 (SPRED1 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. R777-E330 Hs.SPRY2-nostop

   Plasmid

   #70614

   Purpose

   Gateway ORF clone of human SPRY2 [NM_005842.2] without stop codon (for C-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   SPRY2 (SPRY2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R777-E334 Hs.SPRY4-nostop

    Plasmid

    #70617

    Purpose

    Gateway ORF clone of human SPRY4 [NM_001127496.1] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY4 (SPRY4 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSH-Csy4-T2A-SpRFN

    Plasmid

    #85754

    Purpose

    Expresses S. pyogenes FokI-dCas9-NLS with a 25 amino acid linker (GGGGS)5 fusion. Also expresses Csy4 for cleavage of multiplexed gRNA transcripts. Derived from pSQT1601 (Addgene #53369).

    Depositor

    Lawrence Stanton

    Article

    Havlicek et al Mol Ther. 2017 Feb 1;25(2):342-355. doi:

    Insert

    Csy4-T2A-FokI-dCas9
    

    Use

    CRISPR

    Tags

    Csy4 and FokI fusion via 25 amino acid (GGGGS)5 l…

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. R777-E328 Hs.SPRY1-nostop

    Plasmid

    #70612

    Purpose

    Gateway ORF clone of human SPRY1 [NM_001258038.1] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY1 (SPRY1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R777-E324 Hs.SPRED2-nostop

    Plasmid

    #70608

    Purpose

    Gateway ORF clone of human SPRED2 [NM_181784.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRED2 (SPRED2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. R777-E332 Hs.SPRY3-nostop

    Plasmid

    #70616

    Purpose

    Gateway ORF clone of human SPRY3 [NM_005840.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY3 (SPRY3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. L-CRISPR-CTN (MLL-ctrl)

    Plasmid

    #69215

    Purpose

    Advanced lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; control, MLL-sgRNA + luciferase-sgRNA

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    SFFV promoter
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/TO/GFP-SCAF4 (CRISPR_resistant)

    Plasmid

    #122471

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF4 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 (SCAF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO5-SgHottip-GFP-CRISPRa

    Plasmid

    #134990

    Purpose

    dCas9-mediated activation of HOTTIP in mammalian cells

    Depositor

    Suming Huang

    Article

    Luo et al Cancer Cell. 2019 Dec 9;36(6):645-659.e8

    Insert

    HOTTIP (HOTTIP Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. LentiCRISPR v2-sgAMPK alpha1 clone2

    Plasmid

    #162120

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR-Cas9/CD4-TK2

    Plasmid

    #104397

    Purpose

    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

    Depositor

    Jeronimo Blanco

    Article

    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

    Insert

    TK2 (TK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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