We narrowed to 15,538 results for: otos

1. mito-PAGFP

   Plasmid

   #23348

   Purpose

   Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

   Insert

   PAGFP
   

   Use

   Tags

   mito

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG2IG-Hmga1a

   Plasmid

   #122293

   Purpose

   Overexpression for mouse Hmga1a

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Insert

   Hmga1a (Hmga1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET28a-ec_bPAC

   Plasmid

   #28135

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   E.coli adapted photoactivated adenylyl cyclase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-AF8

   Plasmid

   #117803

   Purpose

   mammalian expression of GFP-tagged human centromere protein A

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2000 Aug;25(4):253-61

   Insert

   CENP-A (CENPA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-synPCB2.1

   Plasmid

   #139478

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG2IG-Hmga2

   Plasmid

   #122294

   Purpose

   Overexpression for mouse Hmga2

   Depositor

   Yukiko Gotoh

   Article

   Kishi et al Nat Neurosci. 2012 Aug;15(8):1127-33. do

   Insert

   Hmga2 (Hmga2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CRT-GFP pcDNA3.1

   Plasmid

   #80978

   Purpose

   mammalian expression of CRT-GFP

   Depositor

   Peter Steyger

   Article

   Karasawa et al Toxicol Sci. 2011 Dec;124(2):378-87. doi

   Insert

   CRT (Calr Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   no KDEL

   Promoter

   CMV

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

8. bPAC-mycHis_pGEM

   Plasmid

   #85468

   Purpose

   Expression of photoactivated cyclase bPAC of Beggiatoa sp.

   Depositor

   Peter Hegemann

   Article

   Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

   Insert

   Bacterial Photoactivated Adenylyl Cyclase
   

   Use

   Tags

   His and Myc

   Expression

   Mutation

   Promoter

   T7 promoter

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBXNH3LCA_TM287/288

   Plasmid

   #132701

   Purpose

   expresses TM287/288 in E.coli, to use as positive control in ELISA

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   TM287/288 (TM0288, TM0287 Thermotoga maritima)
   

   Use

   Tags

   Avi and His

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCGN-HCF-1-N1011

    Plasmid

    #92320

    Purpose

    N-term 1-1011 of HCF-1 protein

    Depositor

    Winship Herr

    Article

    Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

    Insert

    HCF-1 (HCFC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    AA 2-1011

    Promoter

    CMV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. spa-GCaMP6s

    Plasmid

    #67556

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6s
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-synPCB2.0

    Plasmid

    #139477

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

    Insert

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    V413L

    Promoter

    CAG promoter

    Available Since

    April 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFIN-IRBP1783-CHER

    Plasmid

    #44357

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    IRBP1783-CHER
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    murine interphotoreceptor retinoid-binding protei…

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sspa-GCaMP6f

    Plasmid

    #67561

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

15. spa-GCaMP6f

    Plasmid

    #67558

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    CMV

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pRSET-spa-GCaMP6f

    Plasmid

    #67563

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 29, 2015

    Availability

    Academic Institutions and Nonprofits only

17. spa-GCaMP6m

    Plasmid

    #67557

    Purpose

    photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6m
    

    Use

    Tags

    HIS-tag

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

18. sspa-GCaMP6m

    Plasmid

    #67560

    Purpose

    short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6m
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available Since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pFIN-IRBP156-tdTOM

    Plasmid

    #44356

    Depositor

    Susan Semple-Rowland

    Article

    Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

    Insert

    IRBP156-tdTOM
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    murine interphotoreceptor retinoid-binding protei…

    Available Since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-TAB-mOvol1

    Plasmid

    #120360

    Purpose

    piggyBac transposon for dox-inducible expression of Ovol1 (with Bgeo; ie. LacZ / NeoR fusion).

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    Ovol1 (Ovol1 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tet-on and CBh

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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