Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 10,627 results for: otos

Clear keywords
Showing: 801 - 820 of 10627 results

  1. pX330_ACTB-e2 sgRNA / hSpCas9

    Plasmid
    #172831
    Purpose
    Mammalian expression of a sgRNA targeting the exon 2 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the exon 2 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172831-plasmid-map-sequence-id-341083

    Enlarge

  2. pX330_Camk2a sgRNA / hSpCas9

    Plasmid
    #172839
    Purpose
    Mammalian expression of a sgRNA targeting the intron 17 (last intron) of Camk2a (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 17 of Camk2a under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    172839-plasmid-map-sequence-id-341073

    Enlarge

  3. pX330_ACTB-i5 sgRNA / hSpCas9

    Plasmid
    #172829
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 5 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172829-plasmid-map-sequence-id-340926

    Enlarge

  4. pX330_ACTB-i3 sgRNA / hSpCas9

    Plasmid
    #172827
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 3 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172827-plasmid-map-sequence-id-340921

    Enlarge

  5. pX330_ACTB-i2 sgRNA / hSpCas9

    Plasmid
    #172826
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 position 2 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172826-plasmid-map-sequence-id-340920

    Enlarge

  6. PB-TAC-OKMS-KRAB-dCas9

    Plasmid
    #120356
    Purpose
    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OKMS cassette
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    Klf4 [10-483]
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120356-plasmid-map-sequence-id-236039

    Enlarge

  7. PB-TAC-OK+9MS-KRAB-dCas9

    Plasmid
    #120357
    Purpose
    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.
    Depositor
    Knut Woltjen
    Article
    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22
    Insert
    OK+9MS cassette
    Use
    CRISPR; Piggybac transposon
    Expression
    Mammalian
    Mutation
    Klf4 [1-483]
    Available Since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    120357-plasmid-map-sequence-id-236025

    Enlarge

  11. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available Since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

    Enlarge

  13. DF-UAS-spa-GCaMP6f

    Plasmid
    #67568
    Purpose
    Zebrafish expression of a photoactivatable fluorescent calcium reporter
    Depositor
    Ehud Isacoff
    Article
    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n
    Insert
    spa-GCaMP6f
    Use
    Zebrafish transgenesis (see :a spinal opsin contr…
    Tags
    HIS-tag
    Mutation
    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…
    Available Since
    Aug. 13, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. iOn-CAG∞RFP

    Plasmid
    #154014
    Purpose
    Vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154014-plasmid-map-sequence-id-308093

    Enlarge

  15. LiOn-CAG∞IRFP

    Plasmid
    #154017
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein IRFP670 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    IRFP670
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154017-plasmid-map-sequence-id-308109

    Enlarge

  16. iOn-CAG∞MCS

    Plasmid
    #154013
    Purpose
    Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    MCS
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154013-plasmid-map-sequence-id-307168

    Enlarge

  20. LiOn-CAG∞GFP

    Plasmid
    #154015
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein EGFP from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Nov. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    154015-plasmid-map-sequence-id-317737

    Enlarge

Showing: 801 - 820 of 10627 results