We narrowed to 5,504 results for: pcas

1. pCA528 CAPN7 (1-75)

   Plasmid

   #193031

   Purpose

   Bacterial expression plasmid for CAPN7 (1-75) N-terminal MIT domain

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   CAPN7 (CAPN7 Human)
   

   Use

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   Residues 1-75

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Expression

   Mutation

   Codon optimized

   Promoter

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-miniCGBE1

   Plasmid

   #198548

   Purpose

   Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NG-miniCGBE1

   Plasmid

   #198538

   Purpose

   Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpCas9-NRRH

   Plasmid

   #198540

   Purpose

   Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRRH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpCas9-NRCH

   Plasmid

   #198542

   Purpose

   Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas3-Apr

   Plasmid

   #208000

   Purpose

   Expresses all necessary components of Type I-C CRISPR-Cas system

   Depositor

   Pablo Ivan Nikel

   Article

   Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

   Inserts

   RhaR (rhaR )
   RhaS (rhaS )
   pRhaBAD
   I-C crRNA
   Cas3
   Cas5
   Cas8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAG_SRGAP2A_GAP(R527L_K566A)

   Plasmid

   #222617

   Purpose

   Expresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.

   Depositor

   Franck Polleux

   Article

   Fossati et al Neuron. 2016 Jul 20;91(2):356-69. doi: 1

   Insert

   SRGAP2 (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutations R527L and K566A (Rac1-specific GA…

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSAVED-CHAT_PCaspase

   Plasmid

   #214043

   Purpose

   Bacterial expression plasmid for SAVED-CHAT and PCaspase N-terminal fragment (aa 1-153) from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSAVED-CHAT_PCaspase_PCi

    Plasmid

    #214049

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase, and PCi from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-Pcaspase-PCi
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT.482

    Plasmid

    #203952

    Purpose

    Spectinomycin-based RSF1010 acceptor Level T

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG_ALX4-Flag-HA

    Plasmid

    #215028

    Purpose

    Mammalian expression of human ALX4

    Depositor

    Joanna Wysocka

    Article

    Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

    Insert

    ALX homeobox 4 (ALX4 Human)
    

    Use

    Tags

    Flag-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-ORF3cStrep

    Plasmid

    #208728

    Purpose

    Expresses ORF3c-Strep in mammalian cells

    Depositor

    Andrew Firth

    Insert

    ORF3c (ORF3a )
    

    Use

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-ORF3cHA

    Plasmid

    #208653

    Purpose

    Express ORF3c-HA in mammalian cells

    Depositor

    Andrew Firth

    Insert

    ORF3c (ORF3a )
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAB1-YFP-GW

    Plasmid

    #209363

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis CAB1 promoter driving expression of N-terminal YFP fusion protein.

    Depositor

    Lucia Strader

    Article

    Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL0_156 [PcatP]

    Plasmid

    #210678

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Insert

    PcatP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LLP781_SnoopCatcher-KRAB-CO

    Plasmid

    #211774

    Purpose

    SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

    Depositor

    Ryan Lister

    Article

    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

    Insert

    Traptavidin-KRAB
    

    Use

    Tags

    3xV5

    Expression

    Mammalian

    Mutation

    Last 300 bp codon-optimised (CO) to detect KRAB e…

    Promoter

    pEF1a and pSV40

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG/ATP11C-c-HA

    Plasmid

    #209222

    Purpose

    Mammalian expression of ATP11C isoform c

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform c (ATP11C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG/ATP11C-b-HA

    Plasmid

    #209221

    Purpose

    Mammalian expression of ATP11C isoform b

    Depositor

    Hye-Won Shin

    Article

    Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

    Insert

    ATP11C isoform b (ATP11C Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. cacnb1b pCAGGS

    Plasmid

    #206102

    Purpose

    expression of rat beta1b calcium channel auxillary subunit

    Depositor

    Annette Dolphin

    Insert

    cacnb1b (Cacnb1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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